剪接体的结构和动力学 - Melissa Moore ParT 2
本视频由科普中国和生物医学大讲堂出品
本节课Melissa Moore讲述了，剪接体的成分和在每个工作周期中的剪接体。穆尔还解释了如何利用荧光蛋白标签和全反射显微镜的创新，使她和她的同事们能够更好地理解复接机器的有序组装与功能。
Melissa Moore (U. Mass/HHMI) ParT 2: Spliceosome STrucTure and Dynamics
Moore goes on To describe The cellular splicing machine, The spliceosome, in greaTer deTail in ParT 2. She lisTs The componenTs of The spliceosome and where each works in The spliceosome cycle. Moore also explains how The innovaTive use of fluorescenT proTein Tags and ToTal inTernal reflecTion microscopy has allowed her and her colleagues To beTTer undersTand The ordered assembly and funcTion of The complex splicing machine.
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生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：hTTp://www.medsci.cn/m/
ConTrolling The Cell Cycle: InTroducTion - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) ParT 1: ConTrolling The Cell Cycle: InTroducTion
Cells reproduce by duplicaTing Their chromosomes and oTher componenTs and Then disTribuTing Them inTo a pair of geneTically idenTical daughTer cells. This series of evenTs is called The cell cycle. In The firsT parT of This lecTure, I provide a general overview of The cell-cycle conTrol sysTem, a complex regulaTory neTwork ThaT guides The cell Through The sTeps of cell division. I briefly describe The major componenTs of This regulaTory sysTem and how They fiT TogeTher To form a series of biochemical swiTches ThaT Trigger cell-cycle evenTs aT The correcT Time and in The correcT order.
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：hTTp://www.medsci.cn/m/
ConTrolling The Cell Cycle: Cdk SubsTraTes - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) ParT 2: ConTrolling The Cell Cycle: Cdk SubsTraTes
Cyclin-dependenT kinases (Cdks) are The cenTral componenTs of The conTrol sysTem ThaT iniTiaTes The evenTs of The cell cycle. In The second parT of This lecTure, I discuss my laboraTory's efforTs To address The problem of how The Cdks Trigger cell-cycle evenTs. I describe our meThods for idenTifying The proTein subsTraTes of The Cdks, and I discuss how These sTudies have led To imporTanT clues abouT how Cdks find Their correcT TargeTs in The cell and how phosphorylaTion of Those TargeTs governs Their funcTion.
ConTrolling The Cell Cycle: Anaphase OnseT - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) ParT 3: ConTrolling The Cell Cycle: Anaphase OnseT
In The anaphase sTage of The cell cycle, The duplicaTed chromosomes are pulled aparT by a machine called The miToTic spindle, resulTing in The disTribuTion of a compleTe seT of chromosomes To each of The daughTer cells. In The Third parT of This lecTure, I describe The combinaTion of biochemisTry and microscopy in my laboraTory ThaT led To The discovery of a regulaTory swiTch ThaT Triggers The abrupT and synchronous separaTion of The chromosomes aT The onseT of anaphase.
PhoTorecepTors and Image Processing ParT 1A - Jeremy NaThans
本视频由科普中国和生物医学大讲堂出品
Jeremy NaThans (Johns Hopkins) ParT 1A: PhoTorecepTors and Image Processing
In This seT of lecTures, Jeremy NaThans explores The molecular mechanisms wiThin The reTina ThaT mediaTe The firsT sTeps in vision. The firsT lecTure focuses on The sTrucTure of The lighT sensing recepTors, The inTracellular signals ThaT are Triggered by lighT absorpTion, and The ways in which The reTina exTracTs informaTion from a complex scene. See more aT hTTp://www.ibioseminars.org
PhoTorecepTors and Image Processing ParT 1B - Jeremy NaThans
本视频由科普中国和生物医学大讲堂出品
Jeremy NaThans (Johns Hopkins) ParT 1B: PhoTorecepTors and Image Processing
In This seT of lecTures, Jeremy NaThans explores The molecular mechanisms wiThin The reTina ThaT mediaTe The firsT sTeps in vision. The firsT lecTure focuses on The sTrucTure of The lighT sensing recepTors, The inTracellular signals ThaT are Triggered by lighT absorpTion, and The ways in which The reTina exTracTs informaTion from a complex scene. See more aT hTTp://www.ibioseminars.org
GE：Dharmacon EdiT-R基因编辑平台——即开即用，任性编辑！
CRISPR-Cas9基因编辑系统正以前所未有的速度席卷科研界。自从GE Dharmacon推出EdiT-R CRISPR-Cas9系统以来，其"无需克隆，任性编辑"的特点广受好评。在此基础上，GE Dharmacon进一步扩充并更新了EdiT-R基因编辑系统，以业界领先的guide RNA设计技术提供各种即开即用的预制产品，同时提供全基因组文库和各信号通路亚文库，满足不同实验者的实验需求。
GE：WesTern bloT 整体解决方案—— Amersham WB与您携手实现完美WB
重复性差、实验周期长、过程繁琐——作为一种最常见蛋白质分析技术，wesTern bloT 在您心中的印象是否依旧如此？wesTern bloT技术于1981年被发明，时至今日，您是否想要了解一下这项技术的最新进展呢？GE公司倾情推出AWB一体化免疫印迹系统，最快4小时完成实验，采用双通道荧光标记技术，并引入总蛋白归一化功能，标准化、自动化流程专注决重复性难题，为您的蛋白质研究保驾护航。
GE：创新解决方案4小时完成wesTern-bloT
WesTern bloT蛋白免疫印迹技术是分子生物学研究的经典研究手段之一，约60％的科学期刊出版物包含WB实验结果。但WB实验步骤多，耗时长，要获得一个完美的结果十分不易，WB实验结果的不稳定，重复不出来？定量不准确？凝胶又漏了？
GE公司最新推出的Amersham WB系统，4小时内可完成电泳、转印、杂交、孵育、成像、定量分析，一气呵成，为WesTern bloT提供了整体解决方案。每个样品、每一次实验都能获得一致的定量数据。再也无需担心结果难以重复再现，无需反复摸索实验条件！
生物学中的蛋白质磷酸化 - Susan Taylor
In This lecTure, I have given an overview of proTein kinase sTrucTure and funcTion using cyclic AMP dependenT kinase (PKA) as a proToType for This enzyme superfamily. I have demonsTraTed whaT we have learned from The overall sTrucTural kinome which allows us To compare many proTein kinases and also To appreciaTe how The highly regulaTed eukaryoTic proTein kinase has evolved. By comparing many proTein kinase sTrucTures, we are beginning To elucidaTe general rules of archiTecTure. In addiTion, I have aTTempTed To illusTraTe how PKA is regulaTed by cAMP and how iT is localized To specific macromolecular complexes Through scaffold proTeins.