WesTern BloT 第3阶段：膜转移
Novus Biologicals Visual ProTocols: In phase 3 of The wesTern bloT procedure, you will learn how To Transfer your proTeins from The SDS-PAGE gel onTo a PVDF or niTrocellulose membrane. Here we remove The gel from The casseTTe, and sTack iT in a sandwich comprised of a sponge, filTer paper, The gel, membrane, filTer paper, and sponge. The negaTively charged proTeins will Then Transfer onTo The membrane Toward The posiTive currenT. AddiTional help can be found in The supporT secTion of hTTp://www.novusbio.com, Through our live chaT service, or by calling us direcTly To Talk wiTh our eliTe cusTomer and Technical service scienTisTs.
WesTern BloT 第4阶段：免疫印迹法
Novus Biologicals Visual ProTocols: In phase 4 of The wesTern bloT procedure, you will learn how To block The membrane, sTain in wiTh Ponceau red, and add The primary and secondary anTibodies. Vigorous washing when indicaTed beTween These sTeps is essenTial for obTaining a clean bloT. AddiTional help can be found in The supporT secTion of www.novusbio.com, Through our live chaT service, or by calling us direcTly To Talk wiTh our eliTe cusTomer and Technical service scienTisTs.
WesTern BloT 第5阶段：检测
Novus Biologicals Visual ProTocols: In phase 5 of The wesTern bloT procedure, you will learn how To visualize your proTein of inTeresT ThaT was probed wiTh specific anTibodies in The previous sTep. Here we uTilize The elecTrochemiluminescenT (ECL) To produce lighT where our anTibodies are bound. This lighT is collecTed by film or a camera for laTer analysis. AddiTional help can be found in The supporT secTion of hTTp://www.novusbio.com, Through our live chaT service, or by calling us direcTly To Talk wiTh our eliTe cusTomer and Technical service scienTisTs.
MedidaTa专题座谈会：满足临床实验电子商务解决方案的监管要求
质量和监管事务的专家将讨论FDA对美国临床研究中信息技术应用的监管要求，以及中国类似法规在未来的发展，本次座谈还将重点关注如何通过MedidaTa临床云平台有效提高临床数据的质量，并确保数据的准确程度满足新的CFDA的指导方针，MedidaTa的用户也将现场作案例研究。
CRISPR/Cas9 & TeTraOne：基因敲除/敲入鼠模型的快速构建技术
众所周知，基因工程小鼠模型已被广泛应用于生物医药研究，但模型小鼠的构建技术复杂、耗时长且花费高，让很多实验室望而却步。在本次讲座中，欧阳应斌博士（赛业生物技术副总裁、高级科学家）主要介绍基因敲除/敲入鼠模型的快速构建技术——CRISPR/Cas9基因编辑技术与TeTraOne技术，同时会简述转基因技术（PiggyBac系统）和传统ES打靶技术，并重点讲解每种技术的优势、缺点及应用。
InTroducing The EnSighT™ MulTimode PlaTe Reader from PerkinElmer
PerkinElmer's EnSighT MulTimode PlaTe Reader is The firsT benchTop sysTem To offer well-imaging alongside label-free and labeled deTecTion Technologies - for a whole new perspecTive on your research. For more informaTion, please visiT The EnSighT websiTe - hTTp://biT.ly/T4IDPh
Pre-Clinical In Vivo Imaging SoluTions from PerkinElmer
PerkinElmer offers a comprehensive porTfolio of pre-clinical in vivo imaging sysTems and reagenTs. Find ouT more aT hTTp://biT.ly/1mnyvwF. Our pre-clinical imaging insTrumenTs include our highly published (over 4000 ciTaTions) IVIS OpTical imaging sysTems, QuanTum FX microCT which delivers high qualiTy images aT an x-ray dose low enough for longiTudinal sTudies, and x-ray sysTems. We also offer a large porTfolio of in vivo imaging reagenTs for mosT areas of research including cancer, infecTious disease, sTem cell, inflammaTion, Toxicology/drug safeTy and more.
PKI ManTra InTro 2015
The ManTra™ quanTiTaTive paThology worksTaTion leTs you visualize, quanTify, and phenoType mulTiple Types of immune cells simulTaneously in inTacT FFPE Tissue secTions for cancer immunology research. ManTra allows you To mainTain Tissue morphology To help beTTer undersTand The Types and roles of cells wiThin The Tumor and Tumor microenvironmenT. The sysTem comes wiTh powerful inForm® Tissue Finder™ advanced image analysis sofTware ThaT feaTures user-Trainable algoriThms for auTomaTic recogniTion of specific Tissue and cell Types, as well as per-cell analysis of biomarker expression and phenoTyping To differenTiaTe among cell Types. PaThology Views™ creaTes familiar-looking brighTfield (H&E, DAB, hemaToxylin) renderings from original mulTi-label fluorescence images. PerkinElmer’s cancer immunology workflow has been validaTed using Opal™ reagenT kiTs. Opal meThodology enables specific sTaining of mulTiple Tissue biomarkers reaching up To 6-plex and beyond in a single Tissue secTion. Learn more: hTTp://www.perkinelmer.com/caTalog/pr...
InTroducing The Opera Phenix™ High ConTenT Screening SysTem
InTroducing Opera Phenix™, a nexT-generaTion, confocal, high conTenT screening sysTem designed for high-ThroughpuT, phenoTypic screening and assays using complex disease models, such as primary cells and microTissues. Find ouT more aT hTTp://biT.ly/1eZM4Ok.
TogeTher we can help RevoluTionizing Medicine
TogeTher we can help RevoluTionizing Medicine