数字PCR技术在HER-2基因扩增检测中的应用
2013年作为PCR技术诞生30周年，本次论坛将围绕PCR技术以及新一代的微滴式数字PCR在肿瘤标记分子领域的应用展开研讨，我们邀请了海内外知名的相关领域卓有建树的科学家采用大会报告以及专题讨论的形式，结合国际上转化医学研究的前沿动态与发展趋势，为大家带来一场生命科学的学术盛宴。
RegenERative medicine for brain and nERve repair
We isolated and propagated neural stem cells from the exposed brain tissue of the patients with open brain trauma, and then implanted neural stem cells with MRI-guided stEReotactic device for the patients. Within 2-years follow-ups, the patients wERe investigated for functional recovERy. Contrast to the case control group, implantation of neural stem cells was associated with a significant improvement in patient's neurological function. Investigations of stem cell thERapy have required analysis of the fate and migration of implanted neural stem cells. HERe, We demonstrate the feasibility of labeling human neural stem cells and retinal stem cells with nanoparticle and tracking of implanted cells in monkey and human central nERvous system (CNS). This data demonstrates the possibility of stem cell thERapy in CNS and collectively provide necessary foundation for ovERcoming challenges to the enhancement of translational regenERative medicine of brain and optic nERve injury.
Study the pathological features of diseases using induced pluripotent stem cells dERived form patient's somatic cells
The limited expERimental access to disease-affected human tissues has sevERely impeded the elucidating of molecular mechanisms undERlying disease development. GenERation of induced pluripotent stem cells (iPSCs) by ovER-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkERs as well as thERapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-dERived cells recapitulated multiple features of pathological events of a particular disease, offERing expERimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current thERapies. We have dERived iPSC lines using somatic cells of patients suffERing from KlinefeltER's Syndrome (KS) and AlzheimER's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
sRNA Induces the Large-scale TransdetERmination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can diffERentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. HowevER, whethER they can transdetERminate into hematopoietic stem cells (HSCs) remains unsolved. We report hERe that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could diffERentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivERed into MSCs by a novel peptide means. These induced MSC-HSCs could form diffERent types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and diffERentiated into all hematopoietic lineages such as ERythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these induced HSCs could successfully engraft and effectively function in patients with sevERe aplastic anemia. FurthERmore, we demonstrated the first evidence that the transdetERmination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. TogethER, our findings identify the sRNAs that dictates a directed diffERentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
The InnER Life of the Cell
由哈佛大学制作的专业视频，描述了从细胞连接、细胞的运动到细胞膜的结构与功能，从细胞骨架到胞内物质的运输，从蛋白质合成到运输与分泌等等，揭示了生命的强大和奇妙。
HERceptin Biosimilar GB221（1）
本课程主要讲述了HERceptin Biosimilar GB221在澳大利亚的1期临床试验的试验背景，相似性研究结果：N-端序列分析，C-端系列分析，色谱分析，等电点，SE-HPLC纯度比较，CE-SDS纯度比较，CEX电荷异构体比较，N-糖分析和比较，亲和力比较，对BT474细胞增殖抑制作用，GB221体外活性比较，GB221药物作用机制研究等一系列知识讲座。
immunothERapy against cancERs(position of sellular thERapy)
Study the pathological features of diseases using induced pluripotent stem cells dERived form patient's somatic cells
The limited expERimental access to disease-affected human tissues has sevERely impeded the elucidating of molecular mechanisms undERlying disease development. GenERation of induced pluripotent stem cells (iPSCs) by ovER-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkERs as well as thERapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-dERived cells recapitulated multiple features of pathological events of a particular disease, offERing expERimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current thERapies. We have dERived iPSC lines using somatic cells of patients suffERing from KlinefeltER's Syndrome (KS) and AlzheimER's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
RegenERative medicine for brain and nERve repair
We isolated and propagated neural stem cells from the exposed brain tissue of the patients with open brain trauma, and then implanted neural stem cells with MRI-guided stEReotactic device for the patients. Within 2-years follow-ups, the patients wERe investigated for functional recovERy. Contrast to the case control group, implantation of neural stem cells was associated with a significant improvement in patient's neurological function. Investigations of stem cell thERapy have required analysis of the fate and migration of implanted neural stem cells. HERe, We demonstrate the feasibility of labeling human neural stem cells and retinal stem cells with nanoparticle and tracking of implanted cells in monkey and human central nERvous system (CNS). This data demonstrates the possibility of stem cell thERapy in CNS and collectively provide necessary foundation for ovERcoming challenges to the enhancement of translational regenERative medicine of brain and optic nERve injury.
免疫印迹（WestERn Blot）操作流程
CST科学家所使用的用于验证抗体的详细的WestERn Blot操作步骤和注意事项，从样本制备直至检测显影。详细展示了关键操作步骤的改变将对实验结果造成不同程度的影响，因此，遵循CST经验证的操作步骤非常重要。