声乐学习与特定的轴突导向基因的表达有关 - ERich Jarvis P3
本视频由科普中国和生物医学大讲堂出品
ERich Jarvis (Duke/HHMI) Part 3: Genes specialized in vocal learning circuits
In Part 1, Jarvis explains that vocal learning is the ability to hear a sound and repeat it. Only 5 groups of mammals (including humans) and 3 groups of birds (parrots, hummingbirds and songbirds) are capable of vocal learning. Jarvis and his lab membERs imaged changes in gene expression in bird's brains aftER singing. They found that hummingbirds, songbirds and parrots each have pathways in specific areas of the brain that are not found in non-vocal learning birds. IntERestingly, analogous networks exist in the human brain but not in non-vocal learning monkeys.
In Part 2, Jarvis proposes a mechanism by which vocal learning may have evolved. He suggests that the brain areas that control vocal learning are the result of a duplication of a pre-existing neural circuit that controls motor movement. A similar duplication event may have occurred during the evolution of humans with the result that both humans and Snowball, a cockatoo, can sing and dance to a beat!
In Jarvis' third talk, he demonstrates that the brain pathways necessary for vocal learning are associated with the expression of particular axonal guidance genes. He also proposes that the evolutionary events responsible for the development of vocal learning may be a genERal mechanism for the development of othER complex behavioral traits.
Photoreceptors and Image Processing Part 1A - JERemy Nathans
本视频由科普中国和生物医学大讲堂出品
JERemy Nathans (Johns Hopkins) Part 1A: Photoreceptors and Image Processing
In this set of lectures, JERemy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellular signals that are triggERed by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibioseminars.org
Photoreceptors and Image Processing Part 1B - JERemy Nathans
本视频由科普中国和生物医学大讲堂出品
JERemy Nathans (Johns Hopkins) Part 1B: Photoreceptors and Image Processing
In this set of lectures, JERemy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellular signals that are triggERed by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibioseminars.org
GE：WestERn blot 整体解决方案—— AmERsham WB与您携手实现完美WB
重复性差、实验周期长、过程繁琐——作为一种最常见蛋白质分析技术，westERn blot 在您心中的印象是否依旧如此？westERn blot技术于1981年被发明，时至今日，您是否想要了解一下这项技术的最新进展呢？GE公司倾情推出AWB一体化免疫印迹系统，最快4小时完成实验，采用双通道荧光标记技术，并引入总蛋白归一化功能，标准化、自动化流程专注决重复性难题，为您的蛋白质研究保驾护航。
GE：创新解决方案4小时完成westERn-blot
WestERn blot蛋白免疫印迹技术是分子生物学研究的经典研究手段之一，约60％的科学期刊出版物包含WB实验结果。但WB实验步骤多，耗时长，要获得一个完美的结果十分不易，WB实验结果的不稳定，重复不出来？定量不准确？凝胶又漏了？
GE公司最新推出的AmERsham WB系统，4小时内可完成电泳、转印、杂交、孵育、成像、定量分析，一气呵成，为WestERn blot提供了整体解决方案。每个样品、每一次实验都能获得一致的定量数据。再也无需担心结果难以重复再现，无需反复摸索实验条件！
如何做一个完美的WestERn Blot检测
In the westERn blot visual protocol video, you will learn how to prepare your samples before loading them into a gel, load a gel and separate the proteins through electrophoresis, transfER your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose membrane, block the membrane, stain in Ponceau red, add the primary and secondary antibodies, and visualize your protein of intERest. Additional help can be found in the support section of http://www.novusbio.com, through our live chat sERvice, or by calling us directly to talk with our elite customER and technical sERvice scientists.
WestERn Blot 第1阶段：样品制备
Novus Biologicals Visual Protocols: In phase 1 of the westERn blot procedure, you will learn how to prepare your samples before loading them into a gel. HERe we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading buffER to the sample, and heat the sample. Additional help can be found in the support section of http://www.novusbio.com, through our live chat sERvice, or by calling us directly to talk with our elite customER and technical sERvice scientists.
WestERn Blot 第2阶段：蛋白电泳(SDS-PAGE)
Novus Biologicals Visual Protocols: In phase 2 of the westERn blot procedure, you will learn how to load a gel and separate the proteins through electrophoresis, based upon protein weight. Additional help can be found in the support section of http://www.novusbio.com, through our live chat sERvice, or by calling us directly to talk with our elite customER and technical sERvice scientists.
WestERn Blot 第3阶段：膜转移
Novus Biologicals Visual Protocols: In phase 3 of the westERn blot procedure, you will learn how to transfER your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose membrane. HERe we remove the gel from the cassette, and stack it in a sandwich comprised of a sponge, filtER papER, the gel, membrane, filtER papER, and sponge. The negatively charged proteins will then transfER onto the membrane toward the positive current. Additional help can be found in the support section of http://www.novusbio.com, through our live chat sERvice, or by calling us directly to talk with our elite customER and technical sERvice scientists.
WestERn Blot 第4阶段：免疫印迹法
Novus Biologicals Visual Protocols: In phase 4 of the westERn blot procedure, you will learn how to block the membrane, stain in with Ponceau red, and add the primary and secondary antibodies. Vigorous washing when indicated between these steps is essential for obtaining a clean blot. Additional help can be found in the support section of www.novusbio.com, through our live chat sERvice, or by calling us directly to talk with our elite customER and technical sERvice scientists.