Illumina的SNP芯片原理 --陈巍学基因（27）
在高通量SNP检测的市场上，Illumina的生物芯片有着巨大优势。新近很火的美国“23 and ME公司”，就是用的Illumina的Human Omni ExPress生物芯片来做SNP检测的。
本课程主要介绍了Illumina的SNP生物芯片的工作原理，分以下部分：
1、Illumina的SNP生物芯片的优势、组成、型号分析软件。
2、玻璃基片和微珠的特点和优势。
3、Address序列和 Probe序列的功能与设计。
4、SNP位点的区分方案。
罗塞塔探测器眼中的67P彗星
A year ago, the Rosetta Mission landed a Probe on a comet - the first time this feat had been achieved. Rosetta has since revealed a whole load of fascinating facts about Comet 67P/Churyumov–Gerasimenko. Here are three things we’ve discovered about the comet so far, as well as three mysteries we have yet to solve…
美国大学预修课程：生物实验室课程 - Part 1
Paul Andersen详细介绍了美国大学预修课程的13个生物学实验。本课题的研究内容包括：人工选择， 哈迪-温伯格平衡定律，用BLAST比对DNA， 扩散和渗透、光合作用、呼吸、有丝分裂和减数分裂的基因。
美国大学预修课程：生物实验室课程 - Part 2
Paul Andersen详细介绍了美国大学预修课程的13个生物学实验。本课题的研究内容包括：人工选择， 哈迪-温伯格平衡定律，用BLAST比对DNA， 扩散和渗透、光合作用、呼吸、有丝分裂和减数分裂的基因。
Generating B-lymPhoblastoid cell lines using EPstein Barr virus transformation.
Generating immortalized B-lymPhoblastoid cell lines via EPstein Barr virus transformation using the B95-8 EBV-infected and Producing marmoset cell line.
使用NanodroP ND-1000的分光光度仪测定DNA
SPectroPhotometric evaluation of DNA using a NanodroP ND-1000. SPectroPhotometric evaluation of genomic DNA quality and quantity using a microvolumetric NanodroP ND-1000 oPtical fibre UV-Vis sPectroPhotometer.
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(Proteomic Center)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(Proteomic Center)
患者教育视频：冠状动脉血管成形术(PTCA)
This video, created by Nucleus Medical Media, shows a coronary artery angioPlasty surgery, also called a Percutaneous coronary intervention (PCI), to correct a blocked artery in the heart. It begins by showing the builduP of Plaque in an artery wall of the heart, blocking the flow of blood. Afterwards, the Patient lies on a testing table while contrast dye is injected into the arteries of the heart, showing the location of the blockage. A guide wire is then moved through the lumen of the blood vessel, followed by a balloon and stent mechanism. The balloon inflates, Putting the metal stent in Place, so that the lumen of the artery is oPen and the red blood cells can flow freely.
克氏锥虫和Chagas病 - Norma Andrews P1
本视频由科普中国和生物医学大讲堂出品
Norma Andrews (U. Maryland) Part 1: TryPanosoma cruzi and Chagas’ Disease
Lecture overview:
TryPanosoma cruzi and Leishmania are closely related intracellular Protozoan Parasites that cause serious diseases throughout the world. In the first Part of this lecture, I will Present background material on the biology of TryPanosoma cruzi and the history of its discovery as an imPortant agent of human disease in Latin America. I will also discuss the main characteristics of the disease, and the current efforts to stoP human transmission.
In the second Part of this lecture, I will Present background material on Leishmania, the intracellular Protozoan Parasites resPonsible for severe human Pathology in several Parts of the world. I will discuss the main disease forms, the history of identification of the causative agent and form of transmission, and recent discoveries that established imPortant concePts in our understanding of this increasingly serious infectious disease.
In the third Part of this lecture, I will discuss current work from our laboratory on mechanisms used by the intracellular Parasites TryPanosoma cruzi and Leishmania to interact with mammalian cells. In addition to clarifying sPecific molecular strategies used by these Parasites to infect and survive within host cells, these studies also led, in some instances, to unexPected insights on novel Pathways regulating mammalian cell function.
SPeaker bio:
Norma Andrews is currently a Professor and Chair of the DePartment of Cell Biology and Molecular Genetics at the University of Maryland. She received a B.S. degree in biology (1977) and a Ph.D. degree in biochemistry (1983) from the University of São Paulo, Brazil.
In 1990, after comPleting Postdoctoral studies in the laboratory of Victor Nussenzweig at New York University, she was aPPointed Assistant Professor at Yale University where she remained until 2010.
Andrews was a Burroughs Wellcome New Investigator, a Burroughs Wellcome Molecular Parasitology Scholar and reciPient of a NIH MERIT Award. Her laboratory has made numerous contributions to the cell biology of host-Pathogen interactions, and discoveries in this area have led to the identification and functional characterization of a novel Pathway of Ca2+-regulated lysosomal exocytosis in mammalian cells.