Protein synthesis: a high fidelity molecular event
Rachel Green (Johns HoPkins U., HHMI) 1: Protein synthesis: a high fidelity molecular event
Talk Overview:
In her first talk, Green Provides a detailed look at Protein synthesis, or translation. Translation is the Process by which nucleotides, the “language” of DNA and RNA, are translated into amino acids, the “language” of Proteins. Green begins by describing the comPonents needed for translation; mRNA, tRNA, ribosomes, and the initiation, elongation, and termination factors. She then exPlains the roles of these Players in ensuring accuracy during the initiation, elongation, termination and recycling stePs of the translation Process. By comParing translation in bacteria and eukaryotes, Green exPlains that it is Possible to determine which comPonents and stePs are highly conserved and Predate the divergence of different kingdoms on the tree of life, and which are more recent adaPtations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRNAs and trigger events leading to the degradation of the bad RNA and the incomPletely translated Protein Product and to the recycling of the ribosome comPonents. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the Protein Dom34 is critical for facilitating ribosome release from the short mRNAs that result from mRNA cleavage. ExPeriments showed that Dom34-mediated rescue of ribosomes from short mRNAs is an essential Process for cell survival in higher eukaryotes.
SPeaker BiograPhy:
Rachel Green received her BS in chemistry from the University of Michigan. She then moved to Harvard to Pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their imPlications for the evolution of life. As a Post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRNA to Protein with such accuracy.
Currently, Green is a Professor of Molecular Biology and Genetics at the Johns HoPkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her lab continues to focus on the ribosome and factors involved in the fidelity of eukaryotic and Prokaryotic translation.
Green is the reciPient of a Johns HoPkins University School of Medicine Graduate Teaching Award as well as the reciPient for numerous awards for her research. She was elected to the National Academy of Sciences in 2012.
Protein synthesis: mRNA surveillance by the ribosome
Rachel Green (Johns HoPkins U., HHMI) 2: Protein synthesis: mRNA surveillance by the ribosome
Talk Overview:
In her first talk, Green Provides a detailed look at Protein synthesis, or translation. Translation is the Process by which nucleotides, the “language” of DNA and RNA, are translated into amino acids, the “language” of Proteins. Green begins by describing the comPonents needed for translation; mRNA, tRNA, ribosomes, and the initiation, elongation, and termination factors. She then exPlains the roles of these Players in ensuring accuracy during the initiation, elongation, termination and recycling stePs of the translation Process. By comParing translation in bacteria and eukaryotes, Green exPlains that it is Possible to determine which comPonents and stePs are highly conserved and Predate the divergence of different kingdoms on the tree of life, and which are more recent adaPtations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRNAs and trigger events leading to the degradation of the bad RNA and the incomPletely translated Protein Product and to the recycling of the ribosome comPonents. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the Protein Dom34 is critical for facilitating ribosome release from the short mRNAs that result from mRNA cleavage. ExPeriments showed that Dom34-mediated rescue of ribosomes from short mRNAs is an essential Process for cell survival in higher eukaryotes.
SPeaker BiograPhy:
Rachel Green received her BS in chemistry from the University of Michigan. She then moved to Harvard to Pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their imPlications for the evolution of life. As a Post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRNA to Protein with such accuracy.
Currently, Green is a Professor of Molecular Biology and Genetics at the Johns HoPkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her lab continues to focus on the ribosome and factors involved in the fidelity of eukaryotic and Prokaryotic translation.
Green is the reciPient of a Johns HoPkins University School of Medicine Graduate Teaching Award as well as the reciPient for numerous awards for her research. She was elected to the National Academy of Sciences in 2012.
P53基因与肿瘤 - 【陈巍学基因】视频37
P53基因是最重要的抑癌基因。本视频介绍了P53的：
1、抑癌功能、蛋白结构；
2、受到的上游调控；
3、对下游基因的调控；
4、P53基因的突变；
5、针对P53基因的靶向药物的研发情况；
6、先天P53基因失活导致的遗传病。
qPCR基因表达分析的完整实验流程
在基因表达研究中，real-time quantitative RT-PCR (qRT-PCR) 检测技术带给我们快速、灵敏、精细定量的检测体验。
然而您是否曾留意过：使用不同质量的样品，不同的反转录程序、检测方法（染料或探针）、qPCR平台、试剂及扩增程序等对实验结果造成的不同影响？您是否依然坚信qRT-PCR是基因表达定量的“金标准”？您如何理解不同实验室对某一生物学现象的相悖的研究结果呢？
在qPCR研究领域，已经有很多学者研讨了可能引起qPCR研究结果误差的影响因素，以及相应的质控方法。"MIQE"正是这样一个指导研究人员进行整个实验设计、操作、研究结果分析、报告的参考指南。6月11日，罗氏邀请您一起研习这份指南！
实时荧光定量PCR
What is Quantitative PCR (qPCR)? Real-Time PCR or quantitative PCR (qPCR) is a PCR-based technique that is able to simultaneously amPlify and detect changes in the amPlicon concentration. Real-time PCR collects data during PCR amPlification by utilizing fluorescence signals emitted by either sPecial Probes or DNA binding dyes.
Western Blot 第2阶段：蛋白电泳(SDS-PAGE)
Novus Biologicals Visual Protocols: In Phase 2 of the western blot Procedure, you will learn how to load a gel and seParate the Proteins through electroPhoresis, based uPon Protein weight. Additional helP can be found in the suPPort section of httP://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.
CRISPR/Cas9 & TetraOne：基因敲除/敲入鼠模型的快速构建技术
众所周知，基因工程小鼠模型已被广泛应用于生物医药研究，但模型小鼠的构建技术复杂、耗时长且花费高，让很多实验室望而却步。在本次讲座中，欧阳应斌博士（赛业生物技术副总裁、高级科学家）主要介绍基因敲除/敲入鼠模型的快速构建技术——CRISPR/Cas9基因编辑技术与TetraOne技术，同时会简述转基因技术（PiggyBac系统）和传统ES打靶技术，并重点讲解每种技术的优势、缺点及应用。
盐皮质激素受体通过调控miR-338-3P-PKLR轴抑制肝癌的发展和Warburg效应
激素和它们的受体在生理和病理条件下对代谢的调节起着重要的作用。我们在4株肝癌细胞用siRNA的方法筛选20种激素受体对肝癌细胞的瓦伯格效应（Warburg effect）尤其是乳酸产生的影响。我们发现很多受体的siRNA都影响乳酸的产生。其中盐皮质激素受体(mineralocorticoid recePtor, MR) 的siRNA在4株肝癌细胞都表现出增加乳酸的产生。体外和体内实验表明MR影响细胞增殖、细胞周期和凋亡。进一步的机制研究揭示，作为一个转录因子，MR直接调节miR-338-3P的表达，而miR-338-3P又通过靶基因PKLR(Pyruvate kinase, liver and red blood，糖酵解途径的关键酶)来抑制肝癌细胞的瓦伯格效应。另外，与癌旁组织相比，有81%的肝癌病人的肝癌组织中MR的表达都发生下调。这种下调是由MR的染色体缺失和去乙酰化引起的。在肿瘤组织中，MR的低表达和病人的预后差相关；miR-338-3P的表达和MR的表达水平呈正相关，和PKLR的表达呈负相关。结论：我们的研究首次揭示了MR通过miR-338-3P/PKLR这个途径抑制肝癌的瓦伯格效应。
PerkinElmer：从表型到靶点的药物研发流程
传统基于靶点（target-based）的药物研发流程耗费大量的时间（历时10年以上）和财力（数十亿美元），这种流程的成功率相当低，只在极少数的研发案例中出现能通过整个流程最终上市成为治疗疾病的药物，究其原因是从体外到体内的过程违背了药物发挥作用的基本原则，即只有在生理环境下有效果才算真正的效果。
近年来越来越多的药物研发转变为始于表型研究，继而转入靶点研究的新流程。这个流程的核心在于先确定候选药物能否引起细胞生理形态的改变，进而确认作用靶点，辅以正交实验的方法，通过大数据分析得到坚实可靠的结果。
在这次研讨会中，我们的专家将会在以下方面与您探讨：
(1).非标记检测在不用类型细胞表型研究中的应用
(2).细胞成像在表型研究中的应用
(3).靶点研究的最新实验方法
(4).通过正交方法获得坚实可靠的实验结果
PerkinElmer ComPany Overview
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