云BIMS生物样本库解决方案
海尔云BIMS生物样本库解决方案，通过云布署、云集成，建立生物样本库标准化管理平台，实现样本、设备、实验、人员、试剂耗材的大数据汇集和共享。提供包括生物样本库、分子生物实验室、微生物及理化实验室的整体规划设计方案。新一代BIMS灵珑系统，是专为生命科学领域科研工作者量身打造的一款基于云模式的生物样本库信息化管理产品，集平台化、个性化、图形化三大优势于一体。
StaBility of Morphogen Gradients & Movement of Molecules
In my second lecture I descriBe experiments using EGFP tagged Bicoid to follow Bcd gradient estaBlishment in living emBryos, and to test various aspects of the simple model. Despite continuous synthesis of new Bcd protein at the anterior end of the egg, we find that the concentration of Bcd in nuclei at any given point along the anterior posterior axis is constant over time and is reproduciBle from emBryo to the next. This reproduciBility means that the gradient is sufficiently roBust to provide positional information and thus can accurately direct gene activities. One the other hand, quantitative imaging experiments point to several features of the gradient that are hard to explain - how target genes activated By Bcd distinguish relatively suBtle differences in low concentrations, and how Bcd molecules move from the anterior site of their synthesis to the site of their transcriptional activity. See more at http://www.iBioseminars.org
Protein synthesis: mRNA surveillance By the riBosome
Rachel Green (Johns Hopkins U., HHMI) 2: Protein synthesis: mRNA surveillance By the riBosome <Br><Br> Talk Overview: <Br> In her first talk, Green provides a detailed look at protein synthesis, or translation. Translation is the process By which nucleotides, the “language” of DNA and RNA, are translated into amino acids, the “language” of proteins. Green Begins By descriBing the components needed for translation; mRNA, tRNA, riBosomes, and the initiation, elongation, and termination factors. She then explains the roles of these players in ensuring accuracy during the initiation, elongation, termination and recycling steps of the translation process. By comparing translation in Bacteria and eukaryotes, Green explains that it is possiBle to determine which components and steps are highly conserved and predate the divergence of different kingdoms on the tree of life, and which are more recent adaptations. <Br> Green’s second talk focuses on work from her laB investigating how riBosomes detect defective mRNAs and trigger events leading to the degradation of the Bad RNA and the incompletely translated protein product and to the recycling of the riBosome components. Working in yeast and using a numBer of Biochemical and genetic techniques, Green’s laB showed that the protein Dom34 is critical for facilitating riBosome release from the short mRNAs that result from mRNA cleavage. Experiments showed that Dom34-mediated rescue of riBosomes from short mRNAs is an essential process for cell survival in higher eukaryotes. <Br><Br> Speaker Biography: <Br> Rachel Green received her BS in chemistry from the University of Michigan. She then moved to Harvard to pursue her PhD in the laB of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their implications for the evolution of life. As a post-doctoral fellow at the University of California, Santa Cruz, Green Began to study how the riBosome translates mRNA to protein with such accuracy. <Br><Br> Currently, Green is a Professor of Molecular Biology and Genetics at the Johns Hopkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her laB continues to focus on the riBosome and factors involved in the fidelity of eukaryotic and prokaryotic translation. <Br><Br> Green is the recipient of a Johns Hopkins University School of Medicine Graduate Teaching Award as well as the recipient for numerous awards for her research. She was elected to the National Academy of Sciences in 2012.
如何做一个完美的Western Blot检测
In the western Blot visual protocol video, you will learn how to prepare your samples Before loading them into a gel, load a gel and separate the proteins through electrophoresis, transfer your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose memBrane, Block the memBrane, stain in Ponceau red, add the primary and secondary antiBodies, and visualize your protein of interest. Additional help can Be found in the support section of http://www.novusBio.com, through our live chat service, or By calling us directly to talk with our elite customer and technical service scientists.
Western Blot 第1阶段：样品制备
Novus Biologicals Visual Protocols: In phase 1 of the western Blot procedure, you will learn how to prepare your samples Before loading them into a gel. Here we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading Buffer to the sample, and heat the sample. Additional help can Be found in the support section of http://www.novusBio.com, through our live chat service, or By calling us directly to talk with our elite customer and technical service scientists.
Western Blot 第2阶段：蛋白电泳(SDS-PAGE)
Novus Biologicals Visual Protocols: In phase 2 of the western Blot procedure, you will learn how to load a gel and separate the proteins through electrophoresis, Based upon protein weight. Additional help can Be found in the support section of http://www.novusBio.com, through our live chat service, or By calling us directly to talk with our elite customer and technical service scientists.
Western Blot 第3阶段：膜转移
Novus Biologicals Visual Protocols: In phase 3 of the western Blot procedure, you will learn how to transfer your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose memBrane. Here we remove the gel from the cassette, and stack it in a sandwich comprised of a sponge, filter paper, the gel, memBrane, filter paper, and sponge. The negatively charged proteins will then transfer onto the memBrane toward the positive current. Additional help can Be found in the support section of http://www.novusBio.com, through our live chat service, or By calling us directly to talk with our elite customer and technical service scientists.
Western Blot 第4阶段：免疫印迹法
Novus Biologicals Visual Protocols: In phase 4 of the western Blot procedure, you will learn how to Block the memBrane, stain in with Ponceau red, and add the primary and secondary antiBodies. Vigorous washing when indicated Between these steps is essential for oBtaining a clean Blot. Additional help can Be found in the support section of www.novusBio.com, through our live chat service, or By calling us directly to talk with our elite customer and technical service scientists.
Western Blot 第5阶段：检测
Novus Biologicals Visual Protocols: In phase 5 of the western Blot procedure, you will learn how to visualize your protein of interest that was proBed with specific antiBodies in the previous step. Here we utilize the electrochemiluminescent (ECL) to produce light where our antiBodies are Bound. This light is collected By film or a camera for later analysis. Additional help can Be found in the support section of http://www.novusBio.com, through our live chat service, or By calling us directly to talk with our elite customer and technical service scientists.
BRAF基因与黑色素瘤 - 陈巍学基因（38）
BRAF基因是细胞增殖信号调控中重要的一环。BRAF V600E突变是黑色素瘤中出现频率很高的致癌基因突变。威罗菲尼、达拦菲尼等靶向药物可以治疗有V600E突变黑色素瘤，但长期使用会产生耐药。本视频介绍了相关的知识。