GE：DhArmAcon siRNA 文库产品应用解决方案
GE DhArmAcon是siRNA全基因组文库的发明者，拥有RNAi产品领域最多的专利和相关知识产权。DhArmAcon pre-defined siRNA文库是目前应用最广泛、客户最值得信赖的siRNA文库。同时，DhArmAcon牵头成立的RNAi全球协议组织（RGI）的60多个知名科研院所成员使用DhArmAcon siRNA文库取得了良好的效果。siRNA文库筛选加速了基因功能的研究，加深了疾病发生机理的研究，并获得了更多新的药物作用机理和潜在药物靶点，已经成为获得高质量研究数据和加速研究进程的必备工具。
GE：Western blot 整体解决方案—— AmershAm WB与您携手实现完美WB
重复性差、实验周期长、过程繁琐——作为一种最常见蛋白质分析技术，western blot 在您心中的印象是否依旧如此？western blot技术于1981年被发明，时至今日，您是否想要了解一下这项技术的最新进展呢？GE公司倾情推出AWB一体化免疫印迹系统，最快4小时完成实验，采用双通道荧光标记技术，并引入总蛋白归一化功能，标准化、自动化流程专注决重复性难题，为您的蛋白质研究保驾护航。
GE：CRISPR-CAs9基因编辑解决方案
CRISPR-CAs9基因编辑系统是最新开发出的基因编辑手段之一，同时因其“前所未有的高效和令人吃惊的简单易用”等特点，愈发成为科学家竞相追逐的热点。但市面常见的CRISPR-CAs9系统实验步骤多，耗时长，甚至需要保证病毒才能转导细胞，要获得理想的结果十分不易。DhArmAcon强大的研发能力还提供在线设计crRNA工具，轻点鼠标即可完成设计，基因编辑一气呵成！
KRAS和肿瘤靶向治疗 - 陈巍学基因（36）
本视频介绍KRAS基因：
1、结构与功能；
2、在肿瘤中的变异；
3、对肿瘤靶向用药的指导作用；
4、设计针对KRAS基因的靶向药物的难点，和已取得的部分进展；
视频时长12分钟，建议在Wifi条件下观看：
生物学中的蛋白质磷酸化 - SusAn TAylor
In this lecture, I hAve given An overview of protein kinAse structure And function using cyclic AMP dependent kinAse (PKA) As A prototype for this enzyme superfAmily. I hAve demonstrAted whAt we hAve leArned from the overAll structurAl kinome which Allows us to compAre mAny protein kinAses And Also to AppreciAte how the highly regulAted eukAryotic protein kinAse hAs evolved. By compAring mAny protein kinAse structures, we Are beginning to elucidAte generAl rules of Architecture. In Addition, I hAve Attempted to illustrAte how PKA is regulAted by cAMP And how it is locAlized to specific mAcromoleculAr complexes through scAffold proteins.
蛋白激酶的结构 - SusAn TAylor
In this lecture, I hAve given An overview of protein kinAse structure And function using cyclic AMP dependent kinAse (PKA) As A prototype for this enzyme superfAmily. I hAve demonstrAted whAt we hAve leArned from the overAll structurAl kinome which Allows us to compAre mAny protein kinAses And Also to AppreciAte how the highly regulAted eukAryotic protein kinAse hAs evolved. By compAring mAny protein kinAse structures, we Are beginning to elucidAte generAl rules of Architecture. In Addition, I hAve Attempted to illustrAte how PKA is regulAted by cAMP And how it is locAlized to specific mAcromoleculAr complexes through scAffold proteins.
蛋白激酶的调控与定位- SusAn TAylor
In this lecture, I hAve given An overview of protein kinAse structure And function using cyclic AMP dependent kinAse (PKA) As A prototype for this enzyme superfAmily. I hAve demonstrAted whAt we hAve leArned from the overAll structurAl kinome which Allows us to compAre mAny protein kinAses And Also to AppreciAte how the highly regulAted eukAryotic protein kinAse hAs evolved. By compAring mAny protein kinAse structures, we Are beginning to elucidAte generAl rules of Architecture. In Addition, I hAve Attempted to illustrAte how PKA is regulAted by cAMP And how it is locAlized to specific mAcromoleculAr complexes through scAffold proteins.
StAbility of Morphogen GrAdients & Movement of Molecules
In my second lecture I describe experiments using EGFP tAgged Bicoid to follow Bcd grAdient estAblishment in living embryos, And to test vArious Aspects of the simple model. Despite continuous synthesis of new Bcd protein At the Anterior end of the egg, we find thAt the concentrAtion of Bcd in nuclei At Any given point Along the Anterior posterior Axis is constAnt over time And is reproducible from embryo to the next. This reproducibility meAns thAt the grAdient is sufficiently robust to provide positionAl informAtion And thus cAn AccurAtely direct gene Activities. One the other hAnd, quAntitAtive imAging experiments point to severAl feAtures of the grAdient thAt Are hArd to explAin - how tArget genes ActivAted by Bcd distinguish relAtively subtle differences in low concentrAtions, And how Bcd molecules move from the Anterior site of their synthesis to the site of their trAnscriptionAl Activity. See more At http://www.ibioseminArs.org
Protein synthesis: A high fidelity moleculAr event
RAchel Green (Johns Hopkins U., HHMI) 1: Protein synthesis: A high fidelity moleculAr event
TAlk Overview:
In her first tAlk, Green provides A detAiled look At protein synthesis, or trAnslAtion. TrAnslAtion is the process by which nucleotides, the “lAnguAge” of DNA And RNA, Are trAnslAted into Amino Acids, the “lAnguAge” of proteins. Green begins by describing the components needed for trAnslAtion; mRNA, tRNA, ribosomes, And the initiAtion, elongAtion, And terminAtion fActors. She then explAins the roles of these plAyers in ensuring AccurAcy during the initiAtion, elongAtion, terminAtion And recycling steps of the trAnslAtion process. By compAring trAnslAtion in bActeriA And eukAryotes, Green explAins thAt it is possible to determine which components And steps Are highly conserved And predAte the divergence of different kingdoms on the tree of life, And which Are more recent AdAptAtions.
Green’s second tAlk focuses on work from her lAb investigAting how ribosomes detect defective mRNAs And trigger events leAding to the degrAdAtion of the bAd RNA And the incompletely trAnslAted protein product And to the recycling of the ribosome components. Working in yeAst And using A number of biochemicAl And genetic techniques, Green’s lAb showed thAt the protein Dom34 is criticAl for fAcilitAting ribosome releAse from the short mRNAs thAt result from mRNA cleAvAge. Experiments showed thAt Dom34-mediAted rescue of ribosomes from short mRNAs is An essentiAl process for cell survivAl in higher eukAryotes.
SpeAker BiogrAphy:
RAchel Green received her BS in chemistry from the University of MichigAn. She then moved to HArvArd to pursue her PhD in the lAb of JAck SzostAk where she worked on designing cAtAlytic RNA molecules And investigAting their implicAtions for the evolution of life. As A post-doctorAl fellow At the University of CAliforniA, SAntA Cruz, Green begAn to study how the ribosome trAnslAtes mRNA to protein with such AccurAcy.
Currently, Green is A Professor of MoleculAr Biology And Genetics At the Johns Hopkins School of Medicine And An InvestigAtor of the HowArd Hughes MedicAl Institute. ReseArch in her lAb continues to focus on the ribosome And fActors involved in the fidelity of eukAryotic And prokAryotic trAnslAtion.
Green is the recipient of A Johns Hopkins University School of Medicine GrAduAte TeAching AwArd As well As the recipient for numerous AwArds for her reseArch. She wAs elected to the NAtionAl AcAdemy of Sciences in 2012.
Protein synthesis: mRNA surveillAnce by the ribosome
RAchel Green (Johns Hopkins U., HHMI) 2: Protein synthesis: mRNA surveillAnce by the ribosome
TAlk Overview:
In her first tAlk, Green provides A detAiled look At protein synthesis, or trAnslAtion. TrAnslAtion is the process by which nucleotides, the “lAnguAge” of DNA And RNA, Are trAnslAted into Amino Acids, the “lAnguAge” of proteins. Green begins by describing the components needed for trAnslAtion; mRNA, tRNA, ribosomes, And the initiAtion, elongAtion, And terminAtion fActors. She then explAins the roles of these plAyers in ensuring AccurAcy during the initiAtion, elongAtion, terminAtion And recycling steps of the trAnslAtion process. By compAring trAnslAtion in bActeriA And eukAryotes, Green explAins thAt it is possible to determine which components And steps Are highly conserved And predAte the divergence of different kingdoms on the tree of life, And which Are more recent AdAptAtions.
Green’s second tAlk focuses on work from her lAb investigAting how ribosomes detect defective mRNAs And trigger events leAding to the degrAdAtion of the bAd RNA And the incompletely trAnslAted protein product And to the recycling of the ribosome components. Working in yeAst And using A number of biochemicAl And genetic techniques, Green’s lAb showed thAt the protein Dom34 is criticAl for fAcilitAting ribosome releAse from the short mRNAs thAt result from mRNA cleAvAge. Experiments showed thAt Dom34-mediAted rescue of ribosomes from short mRNAs is An essentiAl process for cell survivAl in higher eukAryotes.
SpeAker BiogrAphy:
RAchel Green received her BS in chemistry from the University of MichigAn. She then moved to HArvArd to pursue her PhD in the lAb of JAck SzostAk where she worked on designing cAtAlytic RNA molecules And investigAting their implicAtions for the evolution of life. As A post-doctorAl fellow At the University of CAliforniA, SAntA Cruz, Green begAn to study how the ribosome trAnslAtes mRNA to protein with such AccurAcy.
Currently, Green is A Professor of MoleculAr Biology And Genetics At the Johns Hopkins School of Medicine And An InvestigAtor of the HowArd Hughes MedicAl Institute. ReseArch in her lAb continues to focus on the ribosome And fActors involved in the fidelity of eukAryotic And prokAryotic trAnslAtion.
Green is the recipient of A Johns Hopkins University School of Medicine GrAduAte TeAching AwArd As well As the recipient for numerous AwArds for her reseArch. She wAs elected to the NAtionAl AcAdemy of Sciences in 2012.