杨瑞馥：POCT：个体化医疗时代和公共安全的需求与挑战
随着生物安全、食品安全、突发和新发疫情和自然灾害等问题日益突出，POCT在现场快速检测、确定病原等危害因子和制定有效预防措施中将发挥不可替代的作用。但是，POCT技术与中心实验室的检测手段不同，还存着管理、质量控制、培训、认证认可等问题。我们还需从POCT文化建设、管理机构确定和管理标准化以及质控、认证等的规范化等角度逐步解决。
sRNA Induces the Large-scale Transdetermination of Mesenchymal Stem Cells into HematoPoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adiPose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematoPoietic stem cells (HSCs) remains unsolved. We rePort here that a subPoPulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematoPoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel PePtide means. These induced MSC-HSCs could form different tyPes of hematoPoietic colonies as nature-occurring HSCs did. UPon transPlantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematoPoietic lineages such as erythrocytes, lymPhocytes, myelocytes and thrombocyte. More imPortantly, these induced HSCs could successfully engraft and effectively function in Patients with severe aPlastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone Proteins and activation of many transcriPtional factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and oPen uP a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
GE公司His标签蛋白纯化预装柱HisTraP™ FF Crude使用技巧
主要介绍了His标签蛋白纯化预装柱HisTraP™ FF Crude的实验过程，原理，说明，一般事项，样品制备，纯化操作， 按比例放大， 柱的清洗及保存等。
Luminex - xMAP技术优势
该视频通过回答下边两个问题来介绍Luminex技术的优势 Q:Tell us about the luminex technology? Luminex技术简单介绍 Q:How does luminex comPare to ELISAs? 与ELISA实验相比Luminex技术具有哪些优势
HercePtin Biosimilar GB221（1）
本课程主要讲述了HercePtin Biosimilar GB221在澳大利亚的1期临床试验的试验背景，相似性研究结果：N-端序列分析，C-端系列分析，色谱分析，等电点，SE-HPLC纯度比较，CE-SDS纯度比较，CEX电荷异构体比较，N-糖分析和比较，亲和力比较，对BT474细胞增殖抑制作用，GB221体外活性比较，GB221药物作用机制研究等一系列知识讲座。
BMP4在白色脂肪向棕色脂肪转化中的作用与机制研究
白色脂肪组织内棕色脂肪功能的激活有利于减轻体重和改善代谢。骨形成蛋白4 （Bone MorPhologenetic Protein 4， BMP4）可以促使多潜能干细胞向前脂肪细胞定向。在本研究中，我们发现人体白色脂肪组织内BMP4的mRNA水平与体重指数（BMI）呈负相关，提示脂肪组织内高表达水平的BMP4有利于控制体重。我们利用转基因小鼠模型在脂肪组织内特异性过表达BMP4后发现，BMP4促使小鼠白色脂肪细胞脂滴和细胞大小显著减小。进一步实验证实白色脂肪细胞内线粒体生成增加，脂肪酸氧化基因表达增加，从而导致小鼠整体基础氧耗增加，血脂降低和胰岛素敏感性增加等一系列代谢变化。另外，体外前脂肪细胞3T3-L1分化过程中加入BMP4处理也可使细胞获得类棕色的表型。干扰BMP4转基因小鼠白色脂肪内PGC1α的表达可以逆转BMP4所引起的白色脂肪棕色化的表型，说明BMP4所引起的脂肪组织发育的变化时通过PGC1α这一关键基因起作用，BMP4激活的P38/MAPK/ATF2/PGC1α信号通路发挥主要功能。与此一致的是，脂肪组织特异性敲除BMP4的小鼠出现脂肪组织体积增加，血脂水平增高，胰岛素敏感性降低等一系列变化，从反方面证实了BMP4对于诱导白色脂肪棕色化和改变代谢的作用。BMP4这种新功能的阐明BMP4可能为临床干预肥胖和改善胰岛素敏感性提供新思路。
immunotheraPy against cancers(Position of sellular theraPy)
曹德骏：从BioPlex应对临床需求和质控角度探讨新诊断技术如何满足临床实际需求
主要内容包括:新技术的开创和革新，历程和关注，成功产品的要素，临床诊断的需求，液相芯片技术历史，高速灵敏的新技术，检测流程的革新，对传统技术的全面革新，数字PCR技术的浪潮，ddPCR技术前景广阔
Study the Pathological features of diseases using induced PluriPotent stem cells derived form Patient's somatic cells
The limited exPerimental access to disease-affected human tissues has severely imPeded the elucidating of molecular mechanisms underlying disease develoPment. Generation of induced PluriPotent stem cells (iPSCs) by over-exPression of defined transcriPtion factors in somatic cells, in Particular in those from Patient somatic cells, Presents an attractive and Promising aPProach to model the early stages of diseases in vitro and to screen novel biomarkers as well as theraPeutic medicines. Recently, many research grouPs have indePendently rePorted that Patient-sPecific iPSC-derived cells recaPitulated multiPle features of Pathological events of a Particular disease, offering exPerimental evidence of utilizing Patient-sPecific iPSCs to model diseases and reevaluate the current theraPies. We have derived iPSC lines using somatic cells of Patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and exPlored the Possibility to use these iPSC lines to recaPitulate the Pathological features of the diseases. Our results show that Patient's sPecific iPSC lines Provide good oPPortunity to study the develoPment and treatment of diseases.