生物谷会议频道

Regenera<font>T</font>ive medicine for brain and nerve repair

RegeneraTive medicine for brain and nerve repair

We isolaTed and propagaTed neural sTem cells from The exposed brain Tissue of The paTienTs wiTh open brain Trauma, and Then implanTed neural sTem cells wiTh MRI-guided sTereoTacTic device for The paTienTs. WiThin 2-years follow-ups, The paTienTs were invesTigaTed for funcTional recovery. ConTrasT To The case conTrol group, implanTaTion of neural sTem cells was associaTed wiTh a significanT improvemenT in paTienT's neurological funcTion. InvesTigaTions of sTem cell Therapy have required analysis of The faTe and migraTion of implanTed neural sTem cells. Here, We demonsTraTe The feasibiliTy of labeling human neural sTem cells and reTinal sTem cells wiTh nanoparTicle and Tracking of implanTed cells in monkey and human cenTral nervous sysTem (CNS). This daTa demonsTraTes The possibiliTy of sTem cell Therapy in CNS and collecTively provide necessary foundaTion for overcoming challenges To The enhancemenT of TranslaTional regeneraTive medicine of brain and opTic nerve injury.

2014-09-23 课时：48分钟

S<font>T</font>udy <font>T</font>he pa<font>T</font>hological fea<font>T</font>ures of diseases using induced pluripo<font>T</font>en<font>T</font> s<font>T</font>em cells derived form pa<font>T</font>ien<font>T</font>'s soma<font>T</font>ic cells

STudy The paThological feaTures of diseases using induced pluripoTenT sTem cells derived form paTienT's somaTic cells

The limiTed experimenTal access To disease-affecTed human Tissues has severely impeded The elucidaTing of molecular mechanisms underlying disease developmenT. GeneraTion of induced pluripoTenT sTem cells (iPSCs) by over-expression of defined TranscripTion facTors in somaTic cells, in parTicular in Those from paTienT somaTic cells, presenTs an aTTracTive and promising approach To model The early sTages of diseases in viTro and To screen novel biomarkers as well as TherapeuTic medicines. RecenTly, many research groups have independenTly reporTed ThaT paTienT-specific iPSC-derived cells recapiTulaTed mulTiple feaTures of paThological evenTs of a parTicular disease, offering experimenTal evidence of uTilizing paTienT-specific iPSCs To model diseases and reevaluaTe The currenT Therapies. We have derived iPSC lines using somaTic cells of paTienTs suffering from KlinefelTer's Syndrome (KS) and Alzheimer's Disease (AD) and explored The possibiliTy To use These iPSC lines To recapiTulaTe The paThological feaTures of The diseases. Our resulTs show ThaT paTienT's specific iPSC lines provide good opporTuniTy To sTudy The developmenT and TreaTmenT of diseases.

2014-09-25 课时：38分钟

杨瑞馥：POC<font>T</font>：个体化医疗时代和公共安全的需求与挑战

杨瑞馥：POCT：个体化医疗时代和公共安全的需求与挑战

随着生物安全、食品安全、突发和新发疫情和自然灾害等问题日益突出，POCT在现场快速检测、确定病原等危害因子和制定有效预防措施中将发挥不可替代的作用。但是，POCT技术与中心实验室的检测手段不同，还存着管理、质量控制、培训、认证认可等问题。我们还需从POCT文化建设、管理机构确定和管理标准化以及质控、认证等的规范化等角度逐步解决。

2014-09-26 课时：24分钟

sRNA Induces <font>T</font>he Large-scale <font>T</font>ransde<font>T</font>ermina<font>T</font>ion of Mesenchymal S<font>T</font>em Cells in<font>T</font>o Hema<font>T</font>opoie<font>T</font>ic S<font>T</font>em Cells in Human.

sRNA Induces The Large-scale TransdeTerminaTion of Mesenchymal STem Cells inTo HemaTopoieTic STem Cells in Human.

Mesenchymal sTem cells (MSCs) can differenTiaTe inTo cells of bone, endoThelium, adipose Tissue, carTilage, muscle, and brain. However, wheTher They can TransdeTerminaTe inTo hemaTopoieTic sTem cells (HSCs) remains unsolved. We reporT here ThaT a subpopulaTion of human MSCs ThaT are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differenTiaTe inTo hemaTopoieTic sTem cells (CD150+/CD133+/CD34+) and Their descending blood cells in viTro, when TransfecTed wiTh new endogenous shRNAs The sRNA was high-effecTively delivered inTo MSCs by a novel pepTide means. These induced MSC-HSCs could form differenT Types of hemaTopoieTic colonies as naTure-occurring HSCs did. Upon TransplanTaTion inTo subleThally irradiaTed NOD/SCID mice, These MSC-HSCs engrafTed and differenTiaTed inTo all hemaTopoieTic lineages such as eryThrocyTes, lymphocyTes, myelocyTes and ThrombocyTe. More imporTanTly, These induced HSCs could successfully engrafT and effecTively funcTion in paTienTs wiTh severe aplasTic anemia. FurThermore, we demonsTraTed The firsT evidence ThaT The TransdeTerminaTion of MSCs was induced by aceTylaTion of hisTone proTeins and acTivaTion of many TranscripTional facTors. TogeTher, our findings idenTify The sRNAs ThaT dicTaTes a direcTed differenTiaTion of MSCs Toward HSCs and open up a new source for HSCs used for The TreaTmenT of blood diseases and arTificial sTem cell-made blood.