许田博士简介

生物谷

The Xu laboratory is interested in utilizing model organisms to understand the molecular mechanisms of human diseases. We are developing and using new genetic approaches to identify genes that are involved in tumorigenesis, metastasis, neurodegeneration, and hypoxia response, and are exploring the developmental and biochemical functions of these genes in Drosophila, mice, and human cells.

Howard Hughes Medical Institute
Yale University School of Medicine
Department of Genetics
Yale Cancer Center
Room 236
Boyer Center for Molecular Medicine
295 Congress Avenue
New Haven, CT 06536, USA
203-737-2623 (Office)
203-737-2624 (Secretary)
203-737-2621 (Lab)
203-737-1762 (Fax)
tian.xu@yale.edu

BOOKS

Genetic Models in Cardiorespiratory Biology. Ed. Haddad, G. and Xu, T. , Marcel Dekker, Inc. (2001).

Stem Cells and Developmental Biology. Ed. Yi, J.S., Sun, P.F., Tung, X.F. and Xu, T. , M. M. S. Press. (2000).

MANUSCRIPTS

Pagliarini, R.A., and Xu, T. (2003). A genetic screen in Drosophila for metastatic behavior. Science, Oct. 9 Epub ahead of print.

Stewart, R.A., Li, D.M., Huang, H., and Xu, T. (2003). A genetic screen for modifiers of the lats tumor suppressor gene identifies C-terminal Src kinase as a regulator of cell proliferation in Drosophila. Oncogene, 22:6436-6444.

Chen Q, Behar KL, Xu T, Fan C, Haddad GG. (2003). Expression of Drosophila trehalose phosphate synthase in HEK-293 cells increases hypoxia tolerance. J. Biol. Chem., 10: 1074.

Inoki K, Li Y, Xu T, Guan KL. (2003). Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes & Dev. 17:1829-1834.

Pagliarini, R. Quiñones, A., and Xu, T. (2003). Analyzing functions of tumor suppressor genes using a Drosophila model. Methods Mol. Biol., 223:349-382.

Potter CJ, Pedraza LG, Huang H, Xu T. (2003). The tuberous sclerosis complex (TSC) pathway and mechanism of size control. Biochem Soc Trans., 31: 584-586.

Potter CJ, Pedraza LG, Xu T. (2002). Akt regulates growth by directly phosphorylating Tsc2. Nat Cell Biol., 4: 658-665.

Xia H, Qi H, Li Y, Pei J, Barton J, Blackstad M, Xu T, Tao W. (2002). LATS1 tumor suppressor regulates G2/M transition and apoptosis. Oncogene, 21: 1233-1241.

Zhang S, Xu L, Lee J, Xu T. (2002). Drosophila atrophin homolog functions as a transcriptional corepressor in multiple developmental processes. Cell, 108: 45-56.

Chen Q, Ma E, Behar KL, Xu T, Haddad GG. (2002). Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster. J Biol Chem., 277: 3274-3279.

Yang, X.L., Li, D.M., Chen, W.L., and Xu, T. (2001). Human homologue of Drosophila lats, LATS1, negatively regulate growth by inducing G(2)/M arrest or apoptosis. Oncogene, 20:6516-6523.
Turenchalk, G.S. and Xu, T. (2001). Lats in Cell-cycle Regulation and Tumorigenesis. Encyclopedic Reference of Cancer, pp 492-497. Springer.
Potter, C. J. and Xu, T. (2001).Mechanisms of Size Control. Current Opinions in Genetics & Development , 11:279-286.
Potter, C. J., Huang, H. and Xu, T. (2001). Drosophila Tsc1 functions with Tsc2 to antagonize insulin signaling in regulating cell growth, cell proliferation, and organ size. Cell, 105:357-368.
Ma, E., Gu, X.Q., Wu, X.H., Xu, T., and Haddad, G.G. (2001). Mutation in pre-mRNA adenosine deaminase markedly attenuates neuronal tolerance to O2 deprivation in Drosophila melanogaster. J Clin Invest 107:685-693.
Douglas, R.M., Xu, T., and Haddad, G.G. (2001). Cell cycle progression and cell division are sensitive to hypoxia in Drosophila melanogaster embryos. Am J Physiol Regul Integr Comp Physiol 280: R1555-R1563.
Turenchalk, G.S., Potter, C.J., Haddad, G. and Xu, T. (2001). Drosophila as a genetic model system for understanding human biology and disease. Genetic Models in Cardiorespiratory Biology Ed. Haddad, G. and Xu, T., Marcel Dekker, Inc.,
Potter, C.J., Turenchalk, G.S., and Xu, T. (2000). Drosophila in cancer research. An expanding role. Trends Genetics, 16:33-39. Tables for cancer-related genes in Drosophila
Rooke, J.E., Theodosiou, N.A. and Xu, T. (2000). Clonal analysis in the examination of gene function in Drosophila. Methods in Molecular Biology 137: Developmental Biology Protocols III, 15-22.
Xu. R., Cai, J., Xu, T., Zhou, W., Ying B., Deng, K. Zhao, S., and Li, C. (1999). Molecular Cloning and Mapping of A Novel ADAM Gene (ADAM24) to Human Chromosome 4. Genomics, 62:537-9.
Huang, H., Potter, C. J., Tao, W., Li, D.-M., Brogiolo, W., Hafen, E., Sun, H. and Xu, T. (1999). PTEN affects cell size, cell proliferation and apoptosis during Drosophila eye development. Development 126, 5365-5372.
Turenchalk, G.S., St. John, M.A.R., Tao, W., and Xu, T. (1999). The role of Lats in cell cycle regulation and tumorigenesis. Biochimica et Biophysica Acta 1424:M9-M16.
Tao, W., Zhang, S., Turenchalk, G.S., Stewart, R.A., Chang, W., St. John, M.A.R., and Xu, T. (1999). Human homolog of the Drosophila melanogaster lats tumor suppressor modulates CDC2 activity. Nature Genetics, 21:177-181.
St. John, M.A.R., Tao, W., Fei, X., Fukumoto, R., Carcangiu, M.L., Brownstein, D.G., A.F. Parlow, McGrath, J., and Xu, T. (1999). Mice deficient of Lats1 develop soft tissue sarcomas, ovarian tumors and pituitary dysfunction. Nature Genetics, 21:182-186.
Qi, H., Rand, M., Wu, X., Sestan, N., Wang, W., Rakic, P., Xu, T., and Artavanis-Tsakonas, S. (1999). Processing of the Notch ligand Delta by the Metalloprotease Kuzbanian. Science, 283:91-94.
Rooke, J.E. Wu, X.H. and Xu, T. (1999). Cell Interaction During Neural Development. Investigation on Cell Modulation: Signal Transduction, Apoptosis, and Gene Expression.Pp.30-35. Ed. Yi, J.S. et al. Military Medical Science Press (In Chinese).
Ma, E., Xu, T., and Haddad, G.G. (1999) Gene regulation by O2 deprivation: an anoxia-regulated novel gene in Drosophila melanogaster. Molecular Brain Research, 63:217-224.
Theodosiou, N. A., Zhang, S., Wang, W. Y., and Xu, T. (1998). slimb coordinates wg and dpp expression in the dorsal-ventral and anterior-posterior axes during limb development. Development, 125, 3411-3416.
Yavari, R, Adida, C., Brines, M., and Xu, T. (1998). Human Metalloprotease-disintegrin Kuzbanian Regulates Sympathoadrenal Cell Fate in Development and Neoplasia. Human Molecular Genetics, 7:1161-1167.
Theodosiou, N.A. and Xu, T. (1998). Use of the FLP-FRT system to study Drosophila development. Methods, 14,335-365.
Rooke, J.E. and Xu, T. (1998). Positive and negative signals between interacting cells for establishing neuronal fate. Bioessays, 20:209-214.
St. John, M.A.R. and Xu, T. (1997). Understanding Human Cancer In A Fly? American Journal of Human Genetics, 61:1006-1010.
Hu, G., Zhang, S., Vidal, M., Ber, J., Xu, T. and Fearon, E.R. (1997). Mammalian homologs of sina regulate DCC via the ubiquitin-proteasome pathway. Genes & Development, 11:2701-2714.
Haddad, G.G., Sun, Y.-A., Wyman, R.J. and Xu, T. (1997). Genetic basis of tolerance to O2 deprivation in Drosophila melanogaster. Proc. Natl. Acad. Sci. U.S.A., 94:10809-10812.
Rooke, J., Pan, D., Xu, T. and Rubin, G.M. (1996). KUZ, a conserved metalloprotease/disintegrin protein, plays two distinct roles during Drosophila neurogenesis. Science, 273: 1227-1231.
Xu, T., Wang, W., Zhang, S., Stewart, R.A., Yu, W. (1995). Identifying tumor suppressors in genetic mosaics: the Drosophila lats gene encodes a putative protein kinase. Development, 121: 1053-1063.
Xu, T. and Harrison, S. (1994). Mosaic analysis using FLP recombinase. Methods in Cell Biol., 44: 655-682.
Busseau. I., Diederich, R.J., Xu, T. and Artavanis-Tsakonas, S. (1994). A member of the Notch group of interacting loci, deltex encodes a cytoplasmic basic protein. Genetics, 136: 585-596.
Xu, T. and Rubin, G.M. (1993). Analysis of genetic mosaics in developing and adult Drosophila tissues. Development, 117: 1223-1237.
Xu, T., Caron, L. A., Fehon , R.G. and Artavanis-Tsakonas, S. (1992). The involvement of the notch locus in Drosophila oogenesis. Development, 115: 913-922.
Artavanis-Tsakonas, S., Delidakis, C., Fehon, R.G., Hartley, D.A., Herndon, V., Johansen, K.M., Markopoulou, K., Preiss, A., Rebay, I., Scottgale, T.N. and Xu, T. (1990). Notch and the molecular genetics of neuroblast segregation in Drosophila. Mol. Reproduction and Development, 27: 23-27.
Xu, T. and Artavanis-Tsakonas, S. (1990). deltex, a locus interacting with the neurogenic genes, Notch, Delta, and mastermind in Drosophila melanogaster. Genetics, 126: 665-677.
Fehon R.G., Kooh, P.J., Rebay, I., Regan, C.L., Xu, T., Muskavitch, M.A.T. and Artavanis-Tsakonas, S. (1990). Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila . Cell , 61: 523-534.
Xu, T., Rebay, I., Fleming, R.J., Scottgale, T.N. and Artavanis-Tsakonas, S. (1990) The Notch locus and the genetic circuitry involved in early Drosophila neurogenesis. Gen & Devel, 4: 464-475.
Hartley, D.A., Xu, T., Artavanis-Tsakonas, S. (1987). The embryonic expression of the Notch locus of Drosophila melanogaster and the implications of point mutations in the extracellular EGF-like domain of the predicted protein. EMBO J, 6: 3407-3417.
Wharton, K.A., Johansen, K.M., Xu, T., Artavanis-Tsakonas, S. (1985). Nucleotide sequence from the neurogenic locus Notch implies a gene product that shares homology with proteins containing EGF-repeats. Cell ,43: 567-581.
Lin, P.F., Liberman, H.B., Yeh, D.B., Xu, T., Zhao, S.Y., Ruddle, F.H. (1985). Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol. Cell. Biol. , 5: 3149-3156.