蒲慕明博士简介

关键词:ion

Mu-ming Poo

Director
Institute of Neuroscience, CAS
Shanghai 200031
China
Email:mmpoo@ ion.ac.cn
           mpoo@ uclink4.berkeley.edu
Phone: 86-21-5492 1703

Prof. Mu-ming Poo is the founding Director and a Senior Investigator at ION. He is also a co-Principal Investigator of the Laboratory of Neural Plasticity at ION. He currently holds the position of Class of 1933 Professor and Head of Division of Neurobiology at University of California at Berkeley, USA. Professor Poo received his Ph. D. in biophysics from Johns Hopkins University in 1974. His research interests cover many areas in membrane biophysics, synaptic physiology, and developmental neurobiology. In recent years, he has focused his attention on the transduction mechanisms underlying axon guidance and activity-dependent refinement of synaptic connections

Research Interests
We are interested in the cellular and molecular mechanisms underlying the plasticity of developing axons and neural circuits, and the role of neuroplasticity in the development of brain functions.
Ongoing Projects

Plasticity of developing neurons. We are interested in the mechanisms regulating the growth and directional motility of developing axons. Using cultured Xenopus spinal neurons and cerebellar granule cells, we are examining the cytoplasmic events associated with neurite extension and the response of axonal growth cones to extracellular guidance cues. By applying defined extracellular gradients of guidance cues, we can examine the early cellular responses at the growth cone triggered by these molecules and the involvement of various cytoplasmic signaling pathways in regulating the turning decision of the growth cone. In the last two years, we have focused our attention on the role of heterotrimeric G proteins and small Rho family GTPases in mediating the attractive and repulsive turning of the growth cone (See Fig.A). We are currently studying the regulatory mechanisms that modulate the rearrangements of cytoskeletal structures and switch the growth cone turning responses from attraction to repulsion, or vice versa. We are also examining the signal transduction events associated with the repulsive/inhibitory actions of myelin-associated factors, which are known to prevent axon regeneration in the central nervous system after injurye.

Plasticity of neural circuits. Activity-induced changes in the structure and function of neural circuits are responsible for the experience-dependent refinement of developing connections, as well as the learning and memory functions of the brain. We are interested in understanding the effects of patterned electrical activity on the function and connectivity of neural circuits in cultures of dissociated neurons, in acutely isolated brain slices, and in the intact brain. Besides monitoring activity-induced persistent changes in synaptic efficacy, commonly known as long-term potentiation (LTP) and long-term depression (LTD), we have also examined activity-induced changes in the intrinsic neuronal excitability of pre- and the postsynaptic neurons and properties of dendritic integration. We have recently discovered that correlated pre- and postsynaptic activity induces, in addition to LTP or LTD, a marked enhancement or reduction, respectively, of the intrinsic excitability of the presynaptic neuron. Moreover, we found that there is also a significant modification in the linearity of the spatial summation of the potentiated or depressed input with other dendritic inputs on the postsynaptic neuron. These findings indicate that patterned neuronal activity in the neural circuit induces not only persistent modifications of synaptic efficacy, but also global modifications of other properties of the neural circuits critical for information processing. Our current studies are aim to understand the cellular and molecular basis of these circuit modifications and consequence of these modifications on circuit functions.

From neuroplasticity to the development of brain functions. To understanding how circuit modifications induced by patterned neuronal activity influence the integrative functions of the brain, we have chosen the visual system of the Zebrafish as a model system. Using in vivo whole-cell recording of neuronal activity and two-photon imaging of axonal and dendritic morphology of visual neurons in the retinotectal system, we are examining the effects of patterned visual inputs on the refinement of visual circuits. The functional consequences of such refinement will be assessed by the changes in the receptive field properties of these visual neurons. Our goal is to relate neuronal and circuit plasticity to the development of visual functions in the intact developing brain.

Publications

Li, C., Lu, J., Wu, J., Duan, S., and Poo, M. (2004) Bidirectional Modification of Presynaptic Neuronal Excitability Accompanying Spike Timing-Dependent Synaptic Plasticity. Neuron, 41: 257-268

Yang, Y., Ge, W., Chen, Y., Zhang, Z., Shen, W., Wu, C., Poo, M., and Duan, S(2003) Contribution of astrocytes to hippocampal long-term potentiation through release of D-serine. PNAS , 100: 15194-15199.

Zhang, J., Wang, H., Ye, C., Ge, W., Chen, Y,. Jiang, Z., Wu, C., Poo, M., and Duan, S. (2003) ATP Released by Astrocytes Mediates Glutamatergic Activity-Dependent Heterosynaptic Suppression. Neuron, 40: 971-982.

Wang, Z., Xu, N., Wu, C., Duan, S., and Poo, M. (2003) Bidirectional changes in spatial dendritic integration accompanying long-term synaptic modifications. Neuron, 37: 463-472

Yuan, X., Jin, M., Xu, X., Song, Y-q., Wu, C-P., Poo, M-m., and Duan, S-m.(2003), Signaling and crosstalk of rho-GTPases in mediating axon guidance. Nature Cell Biol , 5, 38 - 45 [Abstract [

Note: This paper is cited by Nature Signaling Gateway as a Featured Article.

Xiang Y., Li, Y., Zhang, Z., Cui, K., Wang, S., Yuan, X.B., Wu, C.P., Poo, M-m., Duan, S. (2002)Nerve growth cone guidance mediated by G protein coupled receptors. Nature Neuroscience, 5, 843 - 848 [Abstract[

Zhang, X., and Poo, M. (2002) Localized BDNF-induced synaptic potentiation requires local axonal protein synthesis. Neuron, 36: 675-688 .

Ming, G., Wong, S., Henley, J., Yuan, X., Song, H., Spitzer, N., and M-m. Poo. (2002)Adaptation in the chemotactic guidance of nerve growth cones. Nature 417, 411-8

Engart, F., Tao, H., Zhang, L., and M-m. Poo.(2002). Moving stimuli induces direction-sensitive responses of developing tectal neurons. Nature, 419, 470-475

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