丁玉强（音译）博士简介

生物谷

Yu-Qiang Ding, M.D., Ph.D.

Rm 401, ION Building
Institute of Neuroscience
Chinese Academy of Sciences
Shanghai 200031
China
Phone: 86-21-5492-1773
Fax : 86-21-5492-1735
Email: dingyq@ion.ac.cn

1983.8-1989.7：医学学士，临床医学，西安第四军医大学医疗系（六年制）
1991.8-1993.7: 医学硕士，人体解剖学，西安第四军医大学解剖教研室（提前一年毕业）
1994.2-1996.8: 医学论文博士，神经生物学，日本京都大学医学部高级脑形态学教研室
1996.8-1999.7：医学博士，人体解剖学和组织胚胎学，西安第四军医大学解剖教研室
1989.8-1993.7: 助教，西安第四军医大学解剖教研室
1993.8-1999.7: 讲师，西安第四军医大学解剖教研室
1999.8-2002.12: 副教授，西安第四军医大学神经科学研究所
2000.2-2001.2: 博士后，美国北卡罗莱那医学院细胞生物和解剖学系
2001.3-2004.2: 博士后研究助理，美国圣路易市华盛顿大学麻醉系

目前主要从事神经系统发育的分子机制研究

中枢神经系统内5羟色胺（5-HT）是一种重要的神经递质，与精神情感和镇痛有密切关系，以往的研究已鉴定出若干影响5-HT发育的基因。如鸡胚的移植实验发现，早期移植脊索可异位诱导出5-HT神经元。后来的研究发现脊索的作用是通过Sonic Hedgehog (Shh)实现的，Shh是在时间和空间上决定腹侧神经管神经元产生的重要分子，而5-HT就是在神经管腹侧的产生的。在小鼠, 已知影响5-HT神经元分化成熟的基因为称为GATA3和Pet1两种转录因子, 他们在不同程度上影响5-HT神经元的神经递质的表型。
我们的研究发现一种称为Lmx1b的转录因子在5-HT神经元的分化成熟中起着重要的作用。胚胎发育过程中Lmx1b的表达早于5-HT和Pet1, Lmx1b基因剔除小鼠脑内5-HT和Pet1全部丧失，GATA3表达水平下降, 说明Lmx1b在调控5-HT神经元发育过程中位于GATA3和Pet1的上游, 起着关键的作用。正在进行的研究包括采用组织特异的基因剔除技术，在脑干中缝核内剔除Lmx1b基因, 从而得到没有任何5-HT神经元并能生后存活的小鼠, 用来对5-HT的功能和神经系统发育的作用进行研究。
脊髓是接受躯体多种初级传入信息的第一中枢位点，同时具有明显的板层结构（I-X层），不同的板层接受、整合和传递不同性质的感觉信息。研究脊髓发育的分子机制是神经科学中的一个重要课题。以往此方面的研究多集中在早期脊髓神经管细胞命运决定和背腹模式形成方面。而我们的研究表明这些神经元经迁移分化后分布于脊髓中间带灰质和前角,并不参与脊髓后角的形成。现已知的影响的基因有Lmx1b, Tlx1/Tlx3, Drg11, Lbx1 等。正在进行的研究有通过Microarray技术筛选出脊髓后角特异表达的基因，然后通过基因剔除, RNAi和过度表达等技术来研究这些基因与脊髓后角之间的关系。

Dr. Ding is an Investigator and Head of the Laboratory of Neural Development at ION. He graduated from Fourth Military Medical University (FMMU), Xi'an, China in 1989, and received his Ph. D. from School of Medicine, Kyoto University, Kyoto, Japan in 1996 and from FMMU, Xi'an in 1999. He was a postdoctoral research associate in Aldo Rustioni's lab of Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, NC, USA from 2000 to 2001, and in Zhou-Feng Chen's lab of Department of Anesthesiology, Washington University School of Medicine in St. Louis, MO, USA from 2001 to 2004. His research interest is to study the molecular mechanisms underlying development of the spinal dorsal horn and brainstem.

Research Interests
	His lab is currently focused on the following two projects. First, the study of molecular mechanisms underlying the development of the spinal dorsal horn and the primary projection from the dorsal root ganglia (DRG) to the spinal cord. The dorsal horn is the first site for central processing of the sensory information from the periphery of the body, and some transcription factors (e.g. Drg11, Lbx1, Lmx1b, Pax2, Tlx1/Tlx3) have bee identified as important intrinsic factors controlling cell fate determination, migration, lamination, and differentiation of dorsal horn cells during the development. We are searching for additional genes that are expressed in the developing dorsal horn by microarray and differential screening, and examining their functions in the development. Because the central projection of DRG cells is well-coordinated with the development of the spinal cord, we are also interested in understanding how the central projection of DRG cells is influenced by cell fate determination and differentiation of spinal neurons. The second project deals with the genetic cascade controlling the development of serotonergic neurons in the raphe nuclei of the brainstem and the role of serotonin in the development of the CNS. We have found that Lmx1b is essential for the differentiation of serotonergic neurons, and have roughly delineated the genetic cascade. The current project aims to dissect this cascade in more detail. It has been proposed that serotonin is involved in embryogenesis and morphogenesis. Our interest is to examine the role of serotonin in various aspects of CNS development by using raphe nuclei-specific Lmx1b knockout mice in which no serotonin is synthesized.

	Publications
	Ding YQ, Zhao ZQ, Kania A, Johnson RL & Chen ZF (2004) Lmx1b controls the differentiation and migration of the superficial dorsal horn neurons of the spinal cord. Development (in press) Ding YQ, Marklund U, Yuan W, Yin J, Wegman L, Ericson J, Deneris E, Johson RL, Chen ZF (2003) Lmx1b is essential for the development of serotonergic neurons. Nature Neurosci., 6:933-938. Ding YQ, Yin J, Xu HM, Jackquin MF, Chen ZF (2003) Formation of whisker-related PrV-based lemniscal pathway requires a paired homeodomain transcription factor, Drg11. J Neurosci., 2003, 23:7246-7254. Ding YQ, Lu CR, Wang H, Su CJ, Chen LW, Zhang YQ, Ju G (2002) Two major distinct subpopulations of neurokinin-3 receptor-expressing neurons in the superficial dorsal horn of the rat spinal cord. Eur. J. Neurosci., 16:551-556. Ding YQ, Lu BZ, Guan ZL, Wang DS, Xu JQ, Li JH (1999) Neurokinin B receptor-containing neurons in the paraventricular and supraoptic nuclei of the hypothalamus of the rat synthesize vasopressin and express Fos following intravenous injection of hypertonic saline. Neuroscience, 91:1077-1085. Ding YQ, Zheng HX, Gong LW, Lu Y, Zhao H, Qin BZ (1997) Direct projections from the lumbosacral spinal cord to Barrington's nucleus in the rat: a special reference to micturition reflex. J. Comp. Neurol., 389:149-160. Ding YQ, Kaneko T, Nomura S, Mizuno N (1996) Immunohistochemical localization of -opioid receptor in the central nervous system of the rat. J. Comp. Neurol., 367:375-402. Ding YQ, Shigemoto R, Tadada M, Ohishi H, Nakanishi S, Mizuno N (1996) Localization of neuromedin K receptor in the central nervous system of the rat. J. Comp. Neurol., 364:290-310. Ding YQ, Takada M, Tokuno H, Mizuno N (1995) Direct projections from the dorsolateral potine tegmentum to pudendal motoneurons innervating the exteranl urethral sphincter muscle in the rat. J. Comp. Neurol., 357:318-330.