陈涉博士简介

生物谷

She Chen
Director of Proteomics Facility
National Institute of Biological Sciences
Beijing, China 102206
Tel: 010-80727505
Fax: 010-80727013
E-mail: chenshe@nibs.ac.cn
EDUCATION:
B.S. Biochemistry, June 1995, Wuhan University, China
M.S. Biophysics, June 1998, Institute of Biophysics Chinese Academy of Sciences, Beijing, China
M.S. Food Science and Technology, Dec. 2000, Iowa State University, U.S.A.
EXPERIENCE:
07/2004 – present Director of Proteomics Core Facility, the National Institute of Biological Sciences, Beijing, China
06/2003 – 06/2004 Associate Scientist, MDS Proteomics Inc., Charlottesville, VA, U.S.A.
01/2001– 05/2003 Research Associate, Alliance for Cellular Signaling (AfCS), University of Texas Southwestern Medical Center at Dallas, Dallas, TX, U.S.A.
RESEARCH INTEREST:
蛋白质组中心实验室将重心放在基于生物质谱的蛋白质组研究上，其主要任务和研究方向如下：
Àƒ 为研究所各研究室提供基于生物质谱的蛋白质组平台服务，包括微量蛋白质的鉴定，磷酸化蛋白质磷酸化位点的鉴定，以及蛋白质其它特性的检测．
Àƒ 和需要蛋白质组中心实验室长期或非常规支持的研究室开展合作研究，根据个研究室的要求进行技术平台的开发．
Àƒ 开发和完善对复杂生物样品里的磷酸化蛋白质进行分析的生物学和质谱学平台，包括磷酸化蛋白质或多肽的富集（如IMAC技术），磷酸化位点的确定，以及磷酸化蛋白质的定量．

􀂃 将开发和完善的蛋白质组平台应用于癌症和其它疾病研究，发现新的癌症标记物和药物作用对象．
􀂃 将蛋白质组平台应用于系统生物学的研究领域，鉴定复杂生物系统的信号蛋白和它们的相互作用，建立起复杂生物系统的信号网络，最终促进癌症的研究和新药的发现．
􀂃 Provide routine proteomics service in support of the research work of the institute, which includes: protein sequencing, phosphorylation site identification from individual phosphoprotein, and other protein characterization as requested.
􀂃 Collaborate with other labs of NIBS for critical research projects that require advanced and substantial support from Proteomics Lab.
􀂃 Technology development. The initial effort will be focused on phospho-analysis of complex biological systems and differential labeling of proteins. The Immobilized Metal Affinity Chromatography (IMAC) will be the key technology for phosphopeptide enrichment from complex mixture. The phosphoproteins and their phosphorylation sites will be identified both qualitatively and quantitatively by multiple proteomics platforms.
􀂃 Apply the technology of differential phospho-analysis for cancer research to identify potential cancer markers or drug targets.
􀂃 Integrate various proteomics tools into Systems Biology, identify signaling molecules and build signaling network from complex biological systems, eventually lead to better cancer research and drug discovery.

PUBLICATIONS:
1. Byung Hoon Lee, Xiaoshan Min, Charles J. Heise, Bing Xu, She Chen, Hongjun Shu, Kate Luby Phelps, Elizabeth J. Goldsmith, and Melanie H. Cobb, WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding, Molecular Cell, 2004 (in press)
2. Chen, S., Bobe, G., Zimmerman, S., Hammond, E.G., Luhman, C.M., Boylston, T.D., Freeman, A. E., Beitz, D.C., Physical and sensory properties of dairy products from cows with various milk fatty acid compositions, Journal of Agricultural and Food Chemistry, 2004, 52(11), 3422-3426
3. Hongjun Shu, She Chen, Qun Bi, Marc Mumby, and Deirdre L. Brekken, Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line, Molecular and Cellular Proteomics, 2004, 3(3): 279-286

4. Julia D. Wulfkuhle, Dennis C. Sgroi, Henry Krutzsch, Kelley McLean, Kelly McGarvey, Melodie Knowlton, She Chen, Hongjun Shu, Aysegul Sahin, Raffael Kurek, Diethelm Wallwiener, Maria J. Merino, Emanuel F. Petricoin, III, Yingming Zhao and Patricia S. Steeg, Proteomics of human breast ductal carcinoma in situ, Cancer Research, 2002, 62(22): 6740-6749
5. Ge Zhou, Hongmei Li, Dianne DeCamp, She Chen, Hongjun Shu, Yi Gong, Michael Flag, John Gillespie, Nan Hu, Philip Taylor, Michaeal Emmert Buck, Lance A. Liotta, Emanuel C. Petricoin III, and Yingming Zhao, 2-D differential in-gel electrophoresis for the identification of human esophageal squamous cell cancer specific protein markers, Molecular and Cellular Proteomics, 2002, 1(2): 117-123
6. She Chen, YuSheng Nie, Kunsheng Hu, Study of purinoceptors on polymorphornuclear leukocytes, Progress in Biochemistry and Biophysics, 1999: 26 (4), 366-368
7. She Chen, Yusheng Nie, Kunsheng Hu, Effects of adenosine nucleotides on [Ca2+]i in polymorphornuclear leukocytes, Acta Biophysics, 1997: 210-214