赵光泉博士简介

赵光泉 博士，资深研究员
Guangquan Zhao, Ph.D., Investigator,
National Institute of Biological Sciences, Beijing.
电话（Tel）：010-80726688
传真（Fax）：010-80726689
E-mail：zhaoguangquan@nibs.ac.cn

教育经历Education
1984年 山东医科大学医学部学士,
B.S. Division of Medicine，1979-1984，
Shandong Medical University，Jinan, China
1988年 山东医科大学医学部硕士，
M.S. Division of Medicine，1984-1988，
Shandong Medical University，Jinan, China
1993 年 德克萨斯大学休斯顿健康科学中心生物医学科学博士,
Ph.D. Biomedical Sciences, 1988-1993，
The University of Texas Health Science Center at Houston，Houston, Texas, USA
工作经历Professional Experience
1984-1988 Resident of Surgery，Department of Surgery ，Shandong Medical University Affiliated
Hospital, China（山东医科大学附属医院外科住院医生）
1993-1997 Research Associate, Vanderbilt University Medical School, HHMI and Dept. of Cell
Biology, Nashville, TN（Vanderbilt 大学医学院、霍华德-休斯医学研究所和细胞生
物学系研究助理）
1997-2001 Assistant Professor，Department of Pathobiology，University of Missouri College of
Veterinary Medicine，Columbia, MO（密苏里大学兽医学院病理生物学系助理教授）
2001-present Assistant Professor, Department of Pharmacology & Green Center for Reproductive
Biology, University of Texas Southwestern Medical Center, Harry Hines Blvd.Dallas
（德克萨斯大学西南医学中心药学系和生殖生物学格林中心助理教授）
Reviewer for Journals
Developmental Cell
Development
Developmental Biology
Biology of Reproduction
Endocrinology
Molecular Endocrinology
Journal of Biological Chemistry
Journal of Cell Sciences
Journal of Andrology
Oncogene
Mechanisms of Development
Genesis
European Journal of Endocrinology
University Service
Faculty Judge, University of Missouri Molecular Biology Week; 2000
Reviewer, MU Research Board Awards; 1998,1999, 2000
Faculty Search Committee Chair, UT Southwestern Green Center; 2001-2003
Graduate Student Admissions Committee: 2004-
Public Service
Ad Hoc Reviewer for Catalyst Biomedica Ltd. 2001.
Ad Hoc Reviewer for The Wellcome Trust Funds, 2002.
Ad Hoc Reviewer for NIH Study Section, Dev-1, 2004
研究概述：
多能性胚胎干细胞具有分化为体内各种细胞包括原始生殖细胞(PGC)(所有配子的祖细胞)的能
力。由于PGCs 能在体外培养时形成与胚胎干细胞类似的细胞而也被认为是多能性干细胞。因
此，生殖细胞和多能性具有密切的联系。我们的研究兴趣是干细胞生物学。
1. 研究BMPs 在保持胚胎干细胞多能性中的作用机制：我们已证明BMP4 通过ALK3 受体的信
号传导抑制MAPKs 来保持小鼠胚胎干细胞多能性。为了理解BMPs 在保持多能性中如何起
作用，我们正在通过酵母双杂交系统筛选和共免疫沉淀方法来发现胚胎干细胞中与ALK3 反
应的蛋白质。
2. 研究原始生殖细胞(PGC)的形成机制：虽然我们已经证明BMP4 和BMP8B 的协同作用是PGC
形成所必须的，但在早期胚胎发生中PGC 形成的分子机制还远未研究清楚。我们正在研究
BMP 途径和其他途径是如何配合而导致胚胎干细胞发育为原始生殖细胞。
3. 已分化细胞重编程为多能性干细胞的研究：多能性细胞在组织和细胞治疗中具有巨大的潜
能。已有试验证明胚胎干细胞的胞浆中存在能使已分化的体细胞重编程为多能状态的因子。
如果我们能够利用不同因子的组合将皮肤或血细胞转变为胚胎干细胞，对人类的利益无疑是
巨大的。这是我们的长远目标之一。
4. 研究精原干细胞(SSCs)的自我更新：在男性，PGCs 在胚胎发育过程中进入生殖腺发育成原
精原细胞,并在出生后发育成精原干细胞。精原干细胞具有自我更新及产生分化的精原细胞并
最终形成成熟精子的能力。我们的目标是研究生长因子介导的信号传导途径如何控制在生殖
健康和节育中有重要影响的精原干细胞的自我更新。
Research Description：
The pluripotent embryonic stem (ES) cells have the capacity to differentiate into all cell types of our
body including primordial germ cells (PGCs), the progenitor cells of all gametes. PGCs are also
considered pluripotent stem cells as they can form ES cell-like cells during in vitro culture. Therefore,
germ cells and pluripotency have intimate relationships. Our major research interests are stem cell
biology.
1. To understand how BMPs function to maintain pluripotency of ES cells: We have shown that
BMP4 signaling through ALK3 receptors is required for maintaining the pluripotency of mouse ES
cells through inhibition of MAPKs. To understand how BMP4 functions in maintaining
pluripotency, we are identifying ALK3-interacting proteins in ES cells by yeast two-hybrid screen
and by co-immunoprecipitation.
2. To study the mechanisms of PGC formation: The molecular mechanisms underlying PGC
formation during early embryogenesis are largely unknown although we have shown that the
synergistic actions of BMP4 and BMP8B are required. We are examining how BMP pathways and
other pathways coordinate to direct ES cells into PGCs.
3. To reprogram differentiated cells into pluripotent stem cells:
Pluripotent cells have great potential in tissue and cell therapy. It has been known that the
cytoplasm of an ES cell contains factors capable of reprogramming a differentiated somatic cell
toward a pluripotent state. If we are able to use a combination of different factors to switch our skin
or blood cells into ES cells, the benefit will be enormous. Therefore, one of our long-term goals is
to try to achieve this with human cells.
4. To study the self-renewal of spermatogonial stem cells (SSCs): In males, PGCs become
pro-spermatogonia after they enter gonads during embryogenesis and become SSCs after birth.
SSCs have the ability to self-renew and to generate differentiating spermatogonia to eventually
form mature sperm. Our objectives are to study growth factor-mediated signal pathways in
controlling the self-renewal of SSCs which will have significant impact on reproductive health and
birth control.
发表文章Publications:
1. Zhao, G.-Q., Zhao, Q., Zhou, X., Mattei, M.-G., and de Crombrugghe, B. (1993). TFEC, a basic
helix-loop-helix protein forms heterodimers with TFE3 and inhibits TFE3-dependent
transcriptional activation. Mol. Cell. Biol. 13, 4505-4512.
2. Zhao, G.-Q., Zhou, X., Eberspaecher, H., Solursh, M., and de Crombrugghe, B. (1993). Cartilage
homeoprotein 1, a homeoprotein selectively expressed in chondrocytes. Proc. Natl. Acad. Sci.
USA 90, 8633-8637.
3. Zhao, G.-Q., Zhao, S., Zhou, X., Eberspaecher, H., Solursh, M., and de Crombrugghe, B (1994).
rDlx, a novel Distal-less-like homeoprotein expressed in developing cartilages and discrete
neuronal tissues. Dev. Biol. 164, 37-51.
4. Zhao, G.-Q., Eberspaecher, H., Seldin, M., and de Crombrugghe, B. (1994). The Gene for the
homeodomain-containing protein Cart-1 is expressed in cells that have a chondrogenic potential
during embryonic development. Mech.Dev. 48, 245-254.
5. Zhao, G.-Q. and Hogan, B. L. M. (1996). Evidence that mouse Bmp8a (Op2) and Bmp8b are
duplicated genes that play a role in spermatogenesis and placental development. Mech. Dev. 57,
159-168.
6. Zhao, G.-Q., Deng, K., Labosky, P., Liaw, L., and Hogan, B. L. M. (1996). The gene encoding
bone morphogenetic protein 8B is required for the initiation and maintenance of spermatogenesis
in the mouse. Genes Dev. 10, 1657-1669.
7. Zhao, G.-Q. and Hogan, B. L. M. (1997). Evidence that Mothers-against-dpp-related 1 (Madr1)
plays a role in the initiation and maintenance of spermatogenesis in the mouse. Mech. Dev. 61,
63-73.
8. Brandford, W. W., Zhao, G.-Q., Valerius, M. T., Weinstein, M., Birkenmeier, H., Rowe, L. B.,
and Potter, S. S. (1997). Spx1, a novel X-linked homeobox gene expressed during
spermatogenesis. Mech. Dev. 65, 87-98.
9. Zhao, G.-Q., Liaw, L., and Hogan, B. L. M (1998). Bone Morphogenetic Protein 8A plays a role
in the maintenance of spermatogenesis and integrity of epididymis. Development 125,
1103-1112.
10. Lareyre, J. J., Zheng, W. L., Zhao, G.-Q., Kasper, S., Newcomer, M. E., Matusik, R. J., Ong, D.
E., and Orgebin-Crist, M. C (1998). Molecular cloning and hormonal regulation of a murine
epididymis retinoic acid-binding protein mRNA. Endocrinology 139, 2971-2981.
11. Chen, M.Y., Carpenter, D., and Zhao, G.-Q. (1999) Expression of bone morphogenetic protein
7 in murine epididymis is developmentally regulated. Biol. Reprod. 60, 1503-1508.
12. Wang, R.-A. and Zhao, G.-Q. (1999). TGF- signal transducer Smad2 is expressed in both germ 　
cells and somatic cells during spermatogenesis. Biol. Reprod. 61, 999-1004.
13. Ying, Y., Liu, X.-M., Marble, A., Lawson, K.A., and Zhao, G.-Q. (2000). Requirement of
Bmp8b in the generation of primordial germ cells in the mouse. Mol. Endocrinology 14:
1053-1063.
14. Ying, Y. and Zhao, G.-Q.(2000) Detection of multiple bone morphogenetic protein messenger
ribonucleic acids and their signal transducer, Smad1, during mouse decidualization. Bio. Reprod.
63, 1781-1786.
15. Ying, Y. and Zhao, G.-Q. (2001) Cooperation of extraembryonic ectoderm-derived BMP4 and
endoderm-derived BMP2 in primordial germ cell generation in the mouse. Dev.Biol. 232:
484-492.
16. Ying, Y., Qi, X., and Zhao, G. Q. (2001) Induction of primordial germ cells from murine
epiblasts by synergistic action of BMP4 and BMP8B signaling pathways. Proc. Natl. Acad. Sci.
USA 98, 7858-7862.
17. Zhao, G.-Q., Chen, Y.X., Liu, X., Xu, Z., and Qi, X. (2001) Mutation in Bmp7 exacerbates
phenotype of Bmp8a mutants in Spermatogenesis and Epididymis. Dev. Biol. 240, 212-222.
18. Ying, Y., Qi, X., and Zhao, G.-Q. (2002) Induction of primordial germ cells from pluripotent
epiblasts. The Scientif World Journal 2(3), 801-810.
19. Zhao, G.-Q. and Garbers, D. L. (2002) Male germ cell specification and differentiation . Dev.
Cell 2, 537-547.
20. Yao, H.C., Tilmann., C., Zhao, G.Q., and Capel, B. (2002) The Battle of the sexes: opposing
pathways in sex determination. Novartis Found Symp.244,187-198.
21. Zhao, G.-Q. (2003) Consequences of knocking out BMP signaling in the mouse. genesis 35(1),
43-56.
22. Qi, X., Li, T.G., Hao, J., Hu, J., Wang, J., Simmons, H., Mishina, Y., and Zhao, G.Q. (2004)
BMP4 supports self-renewal of embryonic stem cells by inhibiting mitogen-activated protein
kinase (MAPK) pathways. Proc. Natl. Acad. Sci. USA 101, 6027-6032.
Invited Talks:
Isolation and characterization of cDNAs encoding novel homeodomain proteins from
chondrocytes: University of Iowa, Department of Biology, Iowa City, IA. July 11, 1992.
Isolation and characterization of cDNAs encoding novel homeodomain proteins from
chondrocytes: Vanderbilt University Medical School, Department of Cell Biology, Nashville, TN.
July 7, 1993.
Isolation and characterization of cDNAs encoding novel homeodomain proteins from
chondrocytes: University of Texas Health Science Center at Houston, Graduate School of
Biomedical Sciences, September 20, 1993
Bone Morphogenetic Protein 8A and 8B in spermatogenesis: Vanderbilt University Medical
Center, Center for Reproductive Biology, Nashville, TN, May 7, 1996.
Bone Morphogenetic Protein 8A and 8B in spermatogenesis: University of Minnesota, Center for
Developmental Biology, Minneapolis, MN. May 2, 1996.
Bmp8 genes are required for spermatogenesis in the mouse: Annual Meeting of Society of
Developmental Biology, Nashville, TN, June16, 1996.
Bmp8 genes play a role in germ cell proliferation and survival during spermatogenesis: Gordon
Conferences on Mammalian Gametogenesis and Embryogenesis, NH, August 7, 1996.
Bmp8 genes are required for spermatogenesis in the mouse: University of Missouri, Animal
Sciences Research Center, Columbia, MO. October 20, 1996.
Bmp8 genes are required for spermatogenesis in the mouse: Oregon Primate Center, Beverton,
OR, October 22, 1996.
Bmp8 genes are required for spermatogenesis in the mouse: Baylor College of Medicine,
Department of Cell Biology, Houston, TX, November 2, 1996.
Bone Morphogenetic Proteins in spermatogenesis: Keystone Symposium on Germ Cell
Differentiation, Colorado, March 10-14, 1997.
Roles of BMPs in spermatogenesis: Department of Pharmacology, University of
Missouri-Columbia, Missouri, October 19, 1997.
BMP signaling in mammalian germ cell development: University of Pennsylvania, Center for
Reproduction and Women’s Health, Philadelphia, PA, April 21, 1999.
Roles of BMPs in murine spermatogenesis and primordial germ cell development: National
Institute of Environmental Health Sciences, Research Triangles, NC, May 7, 1999.
BMPs in germ cell development and epididymis maturation: Vanderbilt University Medical
Center, Center for Reproductive Biology, Nashville, TN, May 21, 1999.
Roles of Bone Morphogenetic Proteins in mammalian germ cell development: 32nd Annual
Meeting of the Society for the Study of Reproduction, Pullman, WA, July 31-August 3, 1999.
BMPs are multifunctional regulators of mammalian germ cell development: Chinese Academy of
Sciences, Institute of Zoology and State Key Laboratories in Reproductive Biology, Beijing,
China, November 29, 1999.
BMPs are multifunctional regulators of mammalian germ cell development: University of Kansas
Medical Center, Department of Molecular & Integrative Physiology, Department of Urology,
Department of Anatomy and Cell Biology, Kansas City, KC, December 9, 1999.
BMPs are multifunctional regulators of mammalian germ cell development: Cincinnati
Children’s Hospital Research Foundation, Cincinnati, Ohio, March 6, 2000
BMPs are multifunctional regulators of mammalian germ cell development: University of
Missouri-Columbia, Genetics Area Program, Columbia, Missouri, March 20, 2000
BMPs are multifunctional regulators of mammalian germ cell development: University of
Missouri-Kansas City, Department of Oral Biology, School of Dentistry, Kansas City, Missouri,
April 25, 2000.
BMPs are multifunctional regulators of mammalian germ cell development: University of Hawaii
Medical School, Department of Anatomy and Reproductive Biology, Honolulu, Hawaii, May 17,
2000.
BMPs are multifunctional regulators of mammalian germ cell development: Brown University
Medical School, Women and Infants Hospital of Rhode Island, Providence, RI. July 6, 2000
Bone Morphogenetic Proteins in germ cell generation and development in the mouse: University
of Medicine and Dentistry of New Jersey, Department of Biochemistry and Molecular Biology,
Newark, NJ. August 11, 2000.
BMPs in establishing germ cell lineage in the mouse: University of Texas Southwestern Medical
Center, Cecil H. and Ida Green Center for Reproductive Biology Sciences, Dallas, TX. October
20, 2000.
Induction of primordial germ cells from the epiblast by the synergistic action of BMP4 and
BMP8B signaling pathways: Stem and Progenitor Cells (Biology and Applications), Cold Spring
Harbor Laboratory, NY. March 23, 2001.
Establishment and maintenance of pluripotent stem cells in the mouse. Shandong Academy of
Medical Sciences, Jinan, Shandong, China. November 7, 2001.
Establishment and maintenance of pluripotent stem cells in the mouse. Institute of Embryology,
Shandong University Medical School, Jinan, Shandong, China. November 8, 2001.
Establishment and maintenance of pluripotent stem cells in the mouse. Center for Reproductive
Medicine, Provincial Hospital of Shandong, Jinan, Shandong, China. November 13, 2001.
BMP signaling in the induction of primordial germ cells in the mouse. Department of Molecular
Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka,
Japan. November 16, 2001.
BMP signaling in the induction of primordial germ cells in the mouse. International
Symposium-Development and epigenetics of mammalian germ cells and pluripotent stem cells
(November 19-21, 2001), Kyoto, Japan. November 20, 2001.
BMP signaling in the generation of primordial germ cells of the mouse. Department of Cellular
and Molecular Biology, Baylor College of Medicine, Houston, TX. January 10, 2002.
BMPs in germ cell development and male reproduction. Population Council, Center for
Biomedical Research, New York, NY. November 7, 2002.
Embryonic stem (ES) cells as a tool to study pluripotency and nuclear reprogramming. Center for
Biomedical Inventions, UT Southwestern Medical Center, Dallas, TX. March 18, 2003.
Functional Differences of DPP and 60A Classes of BMPs in Germ Cell Development and Male
Reproduction. XVIIth Testis Workshop-Functional Genomics of Male Reproduction, Phoenix,
AZ, March 26 – 29, 2003.
Molecular Basis of Pluripotency. Institute of Zoology, Chinese Academy of Sciences, Beijing,
China. March 10, 2004.
Molecular Basis of Pluripotency. Shandong University Medical School, Jinan, China. March 16,
2004.
Molecular Basis of Pluripotency. Reproductive Research Institute and Shandong Reproductive
Medical Center, Shandong University, Jinan, China. March 17, 2004.
Molecular Basis of Pluripotency, Shandong Stem Cell Research Center, Yantai, China. April 23,
2004.
Molecular Basis of Pluripotency. MSTP, UT Southwestern Medical Center, Dallas, TX. June 15,
2004.
Current Research Support
NIH (R01HD36218-07): 04/01/98 - 07/31/05. Direct cost: $980,000
(PI: Guang-Quan Zhao).
Genetic Studies of Mammalian Gametogenesis.
NIH (R01 HD37856-04): 06/01/00 - 05/31/05. Direct cost: $675,000
(PI: Guang-Quan Zhao)
BMP signaling in epididymis development and function.
Projects Completed
DOD: 1998.10.1.-2001.2.28. Direct cost: $187,500
(PI: Guang-Quan Zhao).
BMP Signal Transduction in Prostate Development and Carcinogenesis.
March of Dimes: 2000.2.1-2002.1.31. Total Cost: $100,000
(PI: Guang-Quan Zhao)
Genetic studies of male gonocyte development.
NIH (R03 HD39154): 2000.9.1 – 2002. 8. 31. Direct cost: $100,000
(PI: Guang-Quan Zhao)
Mammalian germ cell lineage establishment.
MU Research Board Award: 06/01/97 – 05/031/99 Direct Cost: $50,000
(PI: Guang-Quan Zhao)
Genetic study of mammalian spermatogenesis