新技术：DIY芯片技术
生物谷报道：芯片可以DIY（do-it-yourself)吗？可以的，请看下面报道：
GENETIX |
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Ready-to-go: QArraymini gets you printing. |
76 mm coated slides especially formulated for protein arrays. Proteins arrayed on the nitrocellulose-coated FASTSlide from Schleicher and Schuell Bioscience in Cologne, Germany, are stable for up to three years at room temperature, according to the company. SuperProtein slides coated with hydrophobic polymer from TeleChem International in Sunnyvale, California, and the MaxiSorp black polymer-coated slides from Nalge Nunc in Rochester, New York, are also designed for protein arrays. 3 D HydroGel-coated slides from PerkinElmer in Boston, Massachusetts, provide a hydrophilic environment and the capacity to hold more than a single layer of probes.
To get you started, Genetix in New Milton, UK, has the QArraymini, a ready-to go benchtop microarray spotter. "People can just get it out of the box, and get on with it," says Gail Czerepinski, product manager for Genetix. The QArraymini is available as part of a protein-array package that includes source-plate chiller, buffers, epoxy-coated slides and scanner. The spotter uses contact pin printing to array antibodies or other proteins on 25 75 mm epoxy-coated slides, printing up to 120,000 spots on each slide. "People are printing between a few hundred and a few thousand protein spots. But they can easily scale up should they wish to," says Czerepinski. The aQuire confocal dual-laser scanner, with its own software, images Cy3 and Cy5 dyes simultaneously, with a blue laser fitted as an option.
Protein arrays made in individual wells of 96- or 384-well plastic microplates, such as the plates for the new high-throughput HTAplatform from Greiner Bio-one in Frickhausen and Scienion in Berlin, Germany, are becoming popular, as they allow economical parallel processing of many different test samples. Genetix has developed an optional module for plate arraying for their high-throughput arrayers QArray2 and QArraymax.
L.M.
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