Equipement HPLC Taille image : 275 Ko
	Poste de Biologie Moléculaire Taille image : 282 Ko
	Poste d'Anesthésie Taille image : 321 Ko
	Salle de Neurochimie Taille image : 328 Ko
	Salle de Pré-Culture Taille image : 165 Ko
	Salle de Culture Taille image : 149 Ko
	Poste de Patch-Clamp Taille image : 220 Ko
To characterize mechanosensitive channels functionally we patch-clamp bacteria.  For this purpose we generate giant spheroplasts (1). MscL single-channel currents (2) are activated by suction, they are large (70 pS at 20 mV) and display subconducting states. MscL survives solubilization, purification and reconstitution into liposomes. Panel 3 shows the activation curve recorded at different membrane tensions from a multi-channel liposome patch (from Sukharev, Sigurdson et al., 1999).