 |
CD34+ bone marrow cells were isolated by the use of magnetic beads. These cells were further stained with CFSE, a dye that binds irreversibly to the cells. The cells were sorted by flow cytometry for a narrow distribution of CFSE as well as on lack of glycophorin A expression (upper figure).
These cells were seeded out with the growth factors as indicated on the top for the number of days as indicated to the left. Erythropoietin (EPO) and EPO + stem cell factor (SCF) induced proliferation and differentiation of the cells. These effects were inhibited by LY.
