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Domain binding and function: The HECT domain, short for Homologous to the E6-AP Carboxyl Terminus, is an approximately 40 kDa (350 amino acid) catalytic domain found at the carboxy-terminus of Hect-class E3 ubiquitin protein ligases. This domain functions to bind specific E2s, accepts ubiquitin from the E2 to form a ubiquitin-thioester intermediate with the HECT active cysteine, and then transfers ubiquitin to either the epsilon-amino groups of lysine side chains of the substrate or to the growing end of multiubiquitin chains. The formation of a thioester intermediate with Ub is unique to Hect E3s and has not been observed with other classes of E3s.
Structure Reference: Structure reference: Huang, L. et al. (1999) Science 286 (5443), 1321À1326.
Examples of Domain Proteins:
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| The HECT domain of E6AP ubiquitin-protein ligase. | |
