抗生素溶液配制
信息来源：本站原创 更新时间：2004-7-26 14:20:00
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Working concentrations and stock solutions.
| Antibiotics |
Stock solution |
Working concentration: |
| Strict control |
Weak control |
| mg/ml: |
|
µg/ml |
dilution |
µg/ml |
dilution |
| Ampicillin |
50 (H2O*) |
-20oC |
20 |
0.4 µl/ml |
60-125 |
1.2-2.5 µl/ml |
| Carbenicillin |
50
(H2O, 50%EtOH) |
-20oC |
20 |
0.4 µl/ml |
60-200 |
1.2-4 µl/ml |
| Chloramphenicol |
34
(EtOH, MetOH) |
-20oC |
25 |
0.7 µl/ml |
170 |
5 µl/ml |
| D-cycloserine* |
10
(0.1M NaP pH8.0) |
+4oC |
200 |
20 µl/ml |
|
|
| Gentamycin |
10 (H2O) |
-20oC |
15 |
1.5 µl/ml |
|
|
| Hygromycin B |
10 (H2O) |
-20oC |
10 |
1 µl/ml |
400 |
40 µl/ml |
| Kanamicin |
50 (H2O) |
+4oC-
-20oC |
10 |
0.2 µl/ml |
50 |
1 µl/ml |
| Kasugamycin |
10 (H2O) |
-20oC |
1000 |
100 µl/ml |
|
|
| Nalidixic acid |
5
(H2O, pH to 11 /NaOH) |
-20oC |
15 |
3.0 µl/ml |
|
|
| Neomycin |
10 (H2O) |
-20oC |
100 |
10 µl/ml |
800 |
80 µl/ml |
| Rifampicin** |
34 (MetOH) |
-20oC |
150 |
4.4 µl/ml |
|
|
| Spectinomycin |
10 (H2O) |
-20oC |
100 |
10 µl/ml |
|
|
| Streptomicin |
50 (H2O) |
-20oC |
10 |
0.2 µl/ml |
50 |
1 µl/ml |
| Tetracycline*** |
5 - 12.5
(EtOH, 70%EtOH) |
-20oC |
10 |
2 µl/ml |
50
100 |
10 µl/ml
20 µl/ml | |
* - Unstable solution, prepare just before use;
** - Light sensitive;
*** - Light sensitive, Mg2+ - inhibitor; |
实验频道录入：Protocol 责任编辑：Protocol
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