常用试剂配制－2
信息来源：本站原创 更新时间：2004-7-26 14:00:00
Acetone / Formaldehyde Fixative
Acrylamide Gel, denaturing
Acrylamide Gel, nondenaturing
Alkaline Phosphatase Buffer 1 (Glycine Buffer, 0.1 M, pH 10.4)
Alkaline Phosphatase Buffer 2 (Tris-HCl, 100 mM, pH 9.5)
Alkaline Phospatase Stain (NBT + BCIP)
Amido Black Destain
Ammonium Bicarbonate Buffer, 100x
Annealing Buffer
Antibiotics for Microbiology
Aprotinin Stock Solution
BLOTTO 1 x
Bouins Fixative
Butanol, water saturated
Cadmium Chloride Stock Solution
Calcium Chloride for Competent Cells
Calcium Chloride Stock Solution
CIP Buffer (10x)
Coomassie Destain
Coomassie Stain
Crystal Violet Stock Solution
Cytosol Preparation Buffer
DEAE DNA Elution Buffer I
DEAE DNA Elution Buffer II
DEAE Membrane Wash Buffer
Denhardt's, 50 x
DMEM/MCDB-302
DMEM
DTT Stock Solution
EDTA Stock Solution, 0.5 M
EMSA Binding Buffer
Fischer's Medium (for Nb2 cells)
Glycerol Stock Solution, 80%
HBS (HEPES buffered saline)
HBSS (Hanks Balanced Salt Solution)
HBHA (Hanks Balanced Salt Solution with BSA, HEPES, NaN3)
HeBS (Hepes buffered saline) buffer for electroporation
HEPES Stock Solution
IL-2 Stock Solution
Immunoprecipitation buffer (cell lysis buffer 2)
Immunoprecipitation dilution buffer
Immunoprecipitation wash buffer
IPTG Stock
KCl Stock Solution, 2 M
LB Agar
LB Medium
Lower Tris Buffer, 4x
Magnesium Chloride Stock Solution
MEM
NaCl Stock, 5 M
NaOH Stock, 10 N
NCTC (medium for RCHO-1 cells)
NZY broth
NZY plates
PBS / EDTA solution
PBS Stock Solution, 20x
PIPES Stock Solution
PMSF Stock 0.1 M
R10 (medium for KY1 and KY2 cells)
RIPA buffer (cell lysis buffer 1)
RNK (medium for RNK-16 cells)
RPMI 1640 (medium for YAC-1 cells)
SDS-PAGE, 4 x Running Buffer
SDS-PAGE, 4 x Sample Buffer
SDS Stock Solution, 10%, 20%
SM buffer
SOB-medium
SOC-medium
Sodium Acetate, 3 M
SSC, 20 x
SSPE, 20 x
Stacking Gel
STE, 1 X (for Nuc Trap)
STE for Genomic DNA Isolation
TAE Buffer Stock Solution, 50 x
TBE Stock Solution, 5 x
TBS/TBST buffer, 10x
TDMN Buffer
TE Buffer
Top Agar
Transfer Buffer (for Western Blotting)
Tris Stock Solution, 1 M
Upper Tris Buffer, 4x
Zinc Chloride Stock Solution
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