RNA interference
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Flash Animation: How Does RNAi Work?
Hammond, S.M., Caudy, A.A., Hannon, G.J. (2001) Post-transcriptional Gene Silencing by Double-stranded RNA. Nature Rev Gen 2: 110-119.
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1. Cogoni C, and Macino G. (2000) Post-transcriptional gene silencing across kingdoms. Genes Dev 10: 638-643. (Abstract)
   
   
2. Guru T. (2000). A silence that speaks volumes. Nature 404, 804-808. (Article)
   
   
3. Hammond SM, Caudy AA, Hannon GJ. (2001) Post-transcriptional Gene Silencing by Double-stranded RNA. Nature Rev Gen 2: 110-119. (Abstract)
   
   
4. Napoli C, Lemieux C, and Jorgensen R. (1990) Introduction of a chalcone synthase gene into Petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2: 279-289.
   
   
5. Jorgensen RA, Cluster PD, English J, Que Q, and Napoli CA. (1996) Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences. Plant Mol Biol 31: 957-973. (Abstract)
   
   
6. Ingelbrecht I, Van Houdt H, Van Montagu M, and Depicker A. (1994) Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. Proc Natl Acad Sci USA 91: 10502-10506. (Abstract, PDF of Article)
   
   
7. Cogoni C, Irelan JT, Schumache, M, Schmidhauser T, Selker EU, and Macino G. (1996) Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation. EMBO J 15: 3153-3163. (Abstract)
   
   
8. Palauqui JC, Elmayan T, Pollien JM, and Vaucheret H. (1998) Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J 16: 4738-4745. (Article)
   
   
9. Guo S, and Kempheus KJ. (1995). Par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell 81: 611-620. (Abstract)
   
   
10. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, and Mello CC. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811. (Article)
    
    
11. Timmons, L., and Fire, A. (1998) Specific interference by ingested dsRNA. Nature 395: 854.
    
    
12. Timmons L, Court D, and Fire A. (2001) Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263:103-112. (Abstract)
    
    
13. Hunter CP. (2000) Shrinking the Black Box of RNAi. Current Biology 10: R137-R140. (Article)
    
    
14. Tabara H, Grishok A, and Mello CC. (1998) RNAi in C. elegans: soaking in the genome sequence. Science 282: 430-431.
    
    
15. Kamath RS, Martinez-Campos M, Zipperlen P, Fraser AG, and Ahringer J. (2000) Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biology 2: 2.1-2.10. (Article)
    
    
16. Grishok A, Tabar H, and Mello CC. (2000) Genetic requirements for inheritance of RNAi in C. elegans. Science 287: 2494-2497. (Abstract)
    
    
17. Sharp PA, and Zamore PD. (2000) RNA Interference. Science 287: 2431-2433.
    
    
18. Sharp PA. RNA Interference-2001. (2001) Genes Dev 15: 485-490.
    
    
19. Kennerdell JR, and Carthew RW. (1998) Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell 95: 1017-1026. (Abstract)
    
    
20. Kennerdell JR, and Carthew RW. (2000) Heritable gene silencing in Drosophila using double-stranded RNA. Nature Biotech 18: 896-898. (Abstract)
    
    
21. Dzitoyeva S, Dimitrijevic N, Manev H. (2001) Intra-abdominal injection of double-stranded RNA into anesthetized adult Drosophila triggers RNA interference in the central nervous system. Mol Psychiatry 6(6):665-670.
    
    
22. Worby CA, Simonson-Leff N, Dixon JE. (2001) RNA interference of gene expression (RNAi) in cultured Drosophila cells. Sci STKE Aug 14, 2001(95):PL1.
    
    
23. Schmid A, Schindelholz B, Zinn K. (2002) Combinatorial RNAi: a method for evaluating the functions of gene families in Drosophila. Trends Neurosci 25(2):71-74.)
    
    
24. Hamilton AJ, Baulcombe DC. (1999) A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286: 950-952. (Abstract)
    
    
25. Hammond S, Bernstein E, Beach D, and Hannon G. (2000). An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature, 404: 293-298. (Abstract)
    
    
26. Zamore PD, Tuschl T, Sharp PA, and Bartel DP. (2000). RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101: 25-33. (Abstract)
    
    
27. Hutvagner G, and Zamore PD. (2002) RNAi: nature abhors a double-strand. Curr Opin Genetics & Development 12:225-232.
    
    
28. Bernstein E, Caudy AA, Hammond SA, and Hannon GJ. (2001) Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409:363-366.
    
    
29. Nykanen A, Haley B, and Zamore PD. (2001) ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell 107:309-321.
    
    
30. Lipardi C, Wei Q, and Paterson BM. (2001) RNAi as random degradative PCR. siRNA primers convert mRNA into dsRNA that are degraded to generate new siRNAs. Cell 107:297-307.
    
    
31. Ketting RF, Haverkamp TH, van Luenen HG, and Plasterk RH. (1999). Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNase D. Cell 99: 133-141. (Abstract)
    
    
32. Grishok A, Pasquinelli AE, Conte D, Li N, Parrish S, Ha I, Baillie DL, Fire A, Ruvkun G, and Mello CC. (2001) Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 106:23À34.
    
    
33. Hutvagner G, McLachlan J, Pasquinelli AE, Balint E, Tuschl T, and Zamore PD. (2001) A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293(5531):834-838.
    
    
34. Ketting RF, Fischer SE, Bernstein E, Sijen T, Hannon GJ, and Plasterk RH. (2001) Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev 15(20):2654-2659.
    
    
35. Lagos-Quintana M, Rauhut R, Lendeckel W, and Tuschl T. (2001) Identification of novel genes coding for small expressed RNAs. Science 294:853-858.
    
    
36. Lau NC, Lim LP, Weinstein EG, and Bartel DP. (2001) An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science 294:858-862.
    
    
37. Lee RC, and Ambrose V. (2001) An extensive class of small RNAs in Caenorhabditis elegans. Science 294:862-864.
    
    
38. Ruvkun G. (2001) Glimpses of a tiny RNA world. Science 294:797-799.
    
    
39. Manche L, Green SR, Schmedt C, and Mathews MB. (1992). Interactions between double-stranded RNA regulators and the protein kinase DAI. Mol. Cell. Biol. 12:5238-5248.
    
    
40. Minks MA, West DK, Benvin S, and Baglioni C. (1979). Structural requirements of double-stranded RNA for the activation of 2'-5'-oligo(A) polymerase and protein kinase of interferon-treated HeLa cells. J. Biol. Chem. 254:10180-10183.
    
    
41. Yang S, Tutton S, Pierce E, and Yoon K. (2001) Specific double-stranded RNA interference in undifferentiated mouse embryonic stem cells. Mol. Cell. Biol. 21(22):7807-7816.
    
    
42. Paddison PJ, Caudy A, and Hannon GJ. (2002). Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA 99(3):1443-1448.
    
    
43. Elbashir SM, Lendeckel W, and Tuschl T. (2001) RNA interference is mediated by 21- and 22-nucleotide RNAs Genes Dev 15(2):188-200.
    
    
44. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, and Tuschl T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 494-498. (Abstract)
    
    
45. Caplen NJ, Parrish S, Imani F, Fire A, and Morgan RA. (2001) Specific inhibition of gene expression by small double-stranded RNAs in invertebrates and vertebrate systems. Proc. Natl. Acad. Sci. USA 98: 9746-9747.
    
    
46. Holen T, Amarzguioui M, Wiiger M, Babaie E, and Prydz H. (2002) Positional effects of short interferning RNAs targeting the human coagulation trigger Tissue Factor. Nucleic Acids Research 30(8):1757-1766.
    
    
47. Elbashir SM, Martinez J, Patkaniowska A, Lendeckel W, Tuschl T. (2001) Functional anatomy of siRNA for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J 20: 6877-6888.
    
    
48. Jarvis RA, and Ford LP. (2001) The siRNA Target Site Is an Important Parameter for Inducing RNAi in Human Cells. TechNotes 8(5): 3-5. (Article)
    
    
49. Brown D, Jarvis R, Pallotta V, Byrom M, and Ford L. (2002) RNA Interference in Mammalian Cell Culture: Design, Execution and Analysis of the siRNA Effect. TechNotes 9(1): 3-5. (Article)
    
    
50. Brummelkamp TR, Bernards R, and Agami R. (2002). A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550-553.
    
    
51. Lee NS, Dohjima T, Bauer G, Li H, Li M-J, Ehsani A, Salvaterra P, and Rossi J. (2002). Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nature Biotechnol. 20:500-505.
    
    
52. Miyagishi M, and Taira K. (2002). U6-promoter-driven siRNAs with four uridine 3' overhangs efficiently suppress targeted gene expression in mammalian cells. Nature Biotechnol. 20:497-500.
    
    
53. Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, and Conklin DS. (2002). Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes & Dev. 16:948-958.
    
    
54. Paul CP, Good PD, Winer I, and Engelke DR. (2002). Effective expression of small interfering RNA in human cells. Nature Biotechnol. 20:505-508.
    
    
55. Sui G, Soohoo C, Affar E-B, Gay F, Shi Y, Forrester WC, and Shi Y. (2002). A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 99(6):5515-5520.
    
    
56. Yu J-Y, DeRuiter SL, and Turner DL. (2002). RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99(9):6047-6052.