Western Blotting
信息来源：本站原创 更新时间：2004-8-3 13:02:00
Western Blotting or immunoblotting (Western blotting was dubbed because it is similar to Southern blotting which was invented by and named after the inventor E. M. Southern) allows investigators to determine, with a specific primary antibody, the relative amounts of the protein present in different samples. Briefly, 1) Samples are prepared from tissues or cells that are homogenized in a buffer that protects the protein of interest from degradation; 2) The sample is separated using SDS-PAGE and then transferred to a membrane for detection; 3) The membrane is incubated with a generic protein (such as milk proteins) to bind to any remaining sticky places on the membrane. A primary antibody is then added to the solution which is able to bind to its specific protein; 4) A secondary antibody-enzyme conjugate, which recognizes the primary antibody is added to find locations where the primary antibody bound.
实验频道录入：Protocol 责任编辑：Protocol
- 上一篇实验频道： 没有了
- 下一篇实验频道： Western Blot Protocols
栏目精选
相关文章
没有相关实验频道
文章评论（评论内容只代表网友观点，与生物谷立场无关！）
推荐产品
最新资讯
热点聚焦
推荐文章
