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Assay principles:
- Biotinylated recombinant thermostable luciferase can via streptavidin be linked to any biotinylated primary or secondary antibody. After washing and addition of luciferin and ATP, luminescence output can be immediately recorded in e.g. a microplate luminometer.
- Extremely high sensitivity is obtained by using biotinylated acetate kinase. ADP + acetyl phosphate --------> acetate + ATP Bioluminescent detection of the ATP by the firefly luciferase enzyme can be used to detect the marker enzyme down to 10-21 moles/assay.
Immunoassay products
- InteLite AB for setting up your own immunoassay using thermostable recombinant biotinylated luciferase as marker enzyme
- ZeptoLite AB for setting up your own immunoassay using biotinylated acetate kinase, streptavidin and firefly luciferase. Acetate kinase as marker enzyme can be detected down to 10-21 moles.
- CheckLite for detection of Staphylococcus aureus (result within 7 hours)
Products for hygiene monitoring and rapid microbiology
- Single shot devices LuciPac W and LuciPac II for surface hygiene testing
- CheckLite 250 Plus for detection of bacteria on surfaces or in liquids
- CheckLite HS Set for detection of down to 100 CFU/mL
- CheckLite AM Set for detection of air-borne bacteria
- CheckLite CT for detection of coliforms
- CheckLite Milk for detection of bacteria in milk
- CheckLite Meat for detection of bacteria on meat
Immunoassays
Applications of the biotinylated luciferase or acetate kinase
Suitable for all immunoassays of antigens where a primary antibody is available
- for detection of surface antigens on bacteria
- in receptor studies
- quantification of proteins
ATP / Luciferin
Features
- Very high sensitivity of the luminescent detection
- The high sensitivity reduces the assay time, since the culturing step can be reduced or obviated
- Stable luminescence, suitable for microplate format
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