|
MONITORING OF ENZYMES & METABOLITES
Assay principle
- Any enzyme or metabolite that can be coupled to an ATP producing or degrading reaction can be monitored using the firefly luciferase system
- The analyte can be estimated by endpoint or kinetic analysis.
- ATP Reagent SL enables continuous monitoring of the ATP level simply by measuring the emitted light intensity.
ATP Kit SL
Calibration with internal ATP standard
Applications of the ATP assay
- Oxidative phosphorylation in e.g. diagnosis of inborn metabolic diseases.
- Lipolytic degradation of triacyl-glycerides, in e.g. obesity studies.
- PPi analysis, in e.g. DNA/RNA polymerase activity assays.
- Creatine kinase isoenzyme activity in e.g. diagnosis of acute myocardial infarction.
- ATP/ADP/AMP ratios in e.g. apoptosis studies.
- ATP/phosphocreatine in e.g. muscle metabolism studies.
Features
- Luminometry is often more than 106 times more sensitive than spectrophotometry
- ATP converting reactions can be monitored continuously up to 1 礛 ATP.
- Due to the high sensitivity of the assay, analytical interference from the sample can be obviated by diluting the sample.
- Calibration with internal standard ATP accounts for variations between luminometers, inhibition or quenching of light from the sample and sensitivity of reagents.
|
