The core technology of BioThema AB is analytical applications of the luminescent reaction of the Firefly Luciferase enzyme.
We provide luminescence assay reagents for monitoring of the Luciferase enzyme and ATP.

1. Add 0.1 mL Extractant B/S to a cuvette.
2. Add 0.1 mL sample to the cuvette.
3. Add 0.4 mL reconstituted ATP Reagent HS and measure light emission, I_smp.
4. Add 0.01 mL 10 ^-8 mol/L ATP Standard, i. e. 0.1 pmol ATP, and measure light emission, I_smp+std.

1. The higher the total assay volume the higher the sensitivity of the assay and the more accurate the results.
2. The (I _{smp + std} - I _smp ) value should be at least 10x higher than the I _smp value. If not, the sample should be diluted or a more concentrated ATP standard used.

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