how to determine the chlorophyll content of chloroplasts using a spectrophotometric assay
信息来源：本站原创 更新时间：2004-7-27 10:33:00
| Overview |
| This protocol describes how to determine the chlorophyll content of chloroplasts using a spectrophotometric assay. |
| |
| Procedure |
1. Isolate Chloroplasts from spinach leaves (See protocol for preparation of chloroplasts from spinach leaves).
2. Pipet 1 ml of a chloroplast suspension into a 15 ml centrifuge tube and add 8 ml of Acetone. Mix thoroughly.
3. Add 1 ml of ddH2O, mix again and centrifuge at 1,000 X g for 3 to 5 minutes.
4. Measure the absorbance of the sample from Step 3 on a spectrophotometer at 652 nm (See Hint #1).
5. Calculate the amount of total chlorophyll in 1 ml of the Chloroplast suspension. The amount of chlorophyll is given by the equation:
mg chlorophyll per ml = Absorbance / 34.5
|
| |
| BioReagents and Chemicals |
Acetone
|
| |
| Protocol Hints |
1. Use water as a blank to zero the spectrophotometer.
|
实验频道录入：Protocol 责任编辑：Protocol
文章评论（评论内容只代表网友观点，与生物谷立场无关！）
