LP Recco PCR Cloning Kit
2007-8-6 21:07:53 信息来源：来源网络
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No restriction enzyme, directional cloning of any gene or sequence of interest into any vector
The LP Recco enzyme cloning technology allows cloning your gene or sequence of interest into any vector in one day without the need for restriction enzymes, ligase, or blunt-end polishing.
■Precise, directional cloning of PCR products
■No restriction enzymes required
■No ligase required
■Using any vector you wish to use
■Using any thermostable polymerase for amplification
■Robust performance-easily clone up to 12 kb
■Highest reliability and cloning efficiency
The LP Recco PCR cloning method does not require any restriction enzyme except for liearization of vector. There is no restriction to clone your PCR sequence into any position of the vector you wish to use.
No requirement for presence of A-overhangs, any thermostable polymerase including proof-reading enzyme, such as Vent and Pfu, can be used for amplification,
Using universal actions of The LP Recco enzyme, the LP Recco PCR Cloning Kit provides a fast, high-throughput cloning of your gene or sequence of interest and generation of precise, directional constructs with any vector that are ready for any purpose through incubate for 30 min at room temperature
LP Recco PCR Cloning Kit Contents
LP Recco Enzyme (Lyophilization)
LP Recco Enzyme Dissolution Buffer
10X LP Recco Reaction Buffer
pUC19 (Amp+), Linearized
480-bp Control Insert
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