DNA methylation
信息来源：本站原创 更新时间：2004-7-28 20:13:00
DNA methylation is an epigenetic event that affects cell function by altering gene expression and refers to the covalent addition of a methyl group, catalyzed by DNA methyltransferase (DNMT), to the 5-carbon of cytosine in a CpG dinucleotide. Methods for DNA methylation analysis can be divided roughly into two types: global and gene-specific methylation analysis. For global methylation analysis, there are methods which measure the overall level of methyl cytosines in genome such as chromatographic methods and methyl accepting capacity assay. For gene-specific methylation analysis, a large number of techniques have been developed. Most early studies used methylation sensitive restriction enzymes to digest DNA followed by Southern detection or PCR amplification. Recently, bisulfite reaction based methods have become very popular such as methylation specific PCR (MSP), bisulfite genomic sequencing PCR. Additionally, in order to identify unknown methylation hot-spots or methylated CpG islands in the genome, several of genome-wide screen methods have been invented such as Restriction Landmark Genomic Scanning for Methylation (RLGS-M), and CpG island microarray.
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Methylated CpG Island Amplification
Bisulfite-PCR for Restriction Analysis and/or Sequ
Methylation-Specific PCR
Bisulfite Treatment of DNA
DNA-Methyltransferase Assay
Methylation analyis using restriction enzyme diges
In situ immunodetection of 5-methylcytosine (5-mC)
Singel Nucleotide Primer Extension (SNuPE)
Analysis of methylation using bisulphite sequencin
Bisulfite Modification of DNA
Bisulfite-PCR for Restriction Analysis and/or Sequ
Methylation-Specific PCR
Bisulfite Treatment of DNA
DNA-Methyltransferase Assay
Methylation analyis using restriction enzyme diges
In situ immunodetection of 5-methylcytosine (5-mC)
Singel Nucleotide Primer Extension (SNuPE)
Analysis of methylation using bisulphite sequencin
Bisulfite Modification of DNA
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