Polymerase Chain Reaction

	  				            Sample       Control
================================================== =========== =========== 
distilled water				             40 ul       42 ul
10 X PCR enzyme reaction buffer	                                                        5 ul        5 ul
dNTP mix					             1 ul        1 ul
PCR enzyme			                                  0.25 ul     0.25 ul

						    Sample	 Control
================================================== =========== ===========
downstream primer stock (50 uM)			      1 ul	  1 ul
upstream primer stock (50 uM)			      1 ul	  1 ul
template (at a concentration of 0.5-2 ng/10 ul)	                           2 ul	  0 ul

• 45 seconds at 94 C
• 45 seconds at 60 C
• 2 minutes at 72 C