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Northern杂交
· Southerns, Westerns, and Northerns: Molecular Cloning Techniques (Brian White, MIT)
Basic theory, definition and procedures with illustration
RNA Gel Preparation
· RNA Gel (Crawford Lab)
How to prepare RNA gel
· Glyoxal/DMSO Gel (Carl Clark)
Glyoxal/DMSO gels are useful for determining sizes of RNA especially in conjunction with Northern blots.
Probe Preparation
· Non-radioactive probes (Gottschling Lab)
· Radioactive probes (Gottschling Lab)
Northern Transfer and Hybridization
· Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel (Contributed by Zuyuan Qian)
This is the standard and complete Northern blotting protocol including preparation of RNA gel , RNA sample, and probe, hybridization, detection and recipes for solutions
· Northern Blot Preparation (Tyner Lab)
Detailed protocol for RNA preparation, gel preparation, electrophoresis, gel staining, transfer and cross-linking...
· Northern Transfer Protocol (PMCI Research)
· Northern Blots (Carl Clark)
Detailed protocol for RNA gel, transfer and hybridization
· Northern Blotting (Howell Lab)
Detailed protocol for procedures from RNA isolation, sample preparation, to blot hybridization
· The "Neverfail" Northern Blot Hybridization (NWFSC)
· Northern Blot (Bowtell Lab)
RNA gel and transfer
· Northern Blots ( Michael Koelle) (Ambros Lab)
· Hybridizing Northern Blots with Riboprobes (PMCI Research)
· Northern Blotting (Breeden Lab)
RNA electrophoresis, transfer, crosslink and hybridization...
· Northern Blotting (Crawford Lab)
· Northern Blotting (Gottscheling Lab)
· RNA Dot Blot (Crawford Lab)
· Northern Blotting (Petra Klaff)
complete Northern protocol from RNA gel to blot wash and exposure.
· Filter Hybridisation with Radioactively Labelled Probe: Denhardt Method (Molecular Genetics Network Lab)
Non-isotopic detection
Cross-Linking
· UV Cross-Linking
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