Embryo Buffers
Egg Salts
|
118 mM |
NaCl |
|
48 mM |
KCl |
|
2 mM |
MgCl2 |
|
2 mM |
CaCl2 |
|
10 mM |
HEPES (pH 7.4) |
|
stock soln |
|
|
|
| ||||
|
5 M NaCl |
23.6 |
ul |
236 |
ul |
2.36 |
ml |
118 |
mM |
|
3 M KCl |
16.0 |
ul |
160 |
ul |
1.60 |
ml |
48 |
mM |
|
1 M MgCl2 |
2.0 |
ul |
20 |
ul |
0.20 |
ml |
2 |
mM |
|
1 M CaCl2 |
2.0 |
ul |
20 |
ul |
0.20 |
ml |
2 |
mM |
|
0.5 M HEPES (7.4) |
20.0 |
ul |
200 |
ul |
2.00 |
ml |
10 |
mM |
|
H2O |
936.4 |
ul |
9364 |
ul |
93.64 |
ml |
||
Embryo Growth Medium
|
0.9 X |
Schneider's Drosophila Medium |
|
|
0.5 mg/ml |
Inulin (Sigma I-3754) |
|
|
5.0 mg/ml |
PVP-powder (Sigma P-0930) |
|
|
1 X |
BME vitamin solution (GIBCO 21040-050) |
|
|
1 X |
Defined lipid concentrate (GIBCO 11905-031) |
|
|
1 X |
Penicillin/Streptomycin solution (GIBCO 15070-030) |
|
|
*** mix solution at approximately 65:35 ratio with FBS *** |
||
|
|
|
|
| |||||
|
Schneider's Medium |
9 |
ml |
45 |
ml |
90 |
ml |
0.9 |
|
|
Inulin |
5 |
mg |
25 |
mg |
50 |
mg |
0.5 |
|
|
PVP |
50 |
mg |
250 |
mg |
500 |
mg |
5 |
|
|
100X BME vit. soln. |
0.1 |
ml |
0.5 |
ml |
1 |
ml |
1 |
|
|
100X Lipid concentrate |
0.1 |
ml |
0.5 |
ml |
1 |
ml |
1 |
|
|
100X Pen/Str solution |
0.1 |
ml |
0.5 |
ml |
1 |
ml |
1 |
|
|
H2O |
0.7 |
ml |
3.5 |
ml |
7 |
ml |
||
compiled by Chad Rappleye
Aroian Lab Protocols
