|
Antigen |
Fixation |
Fixation time |
|
|
|
|
microtubules |
100% MeOH |
5 min @ RT |
|
|
|
|
actin |
4% Formaldehyde* |
8 min @ RT |
|
|
|
|
POD-1
|
Formaldehyde or MeOH* |
8 min @ RT |
|
|
|
|
PAR-1 |
100% MeOH |
15 min @ -20 deg |
|
|
|
|
PAR-3 |
100% MeOH |
15 min @ RT |
|
|
|
|
P-granules |
100% MeOH** |
10 min @ 4 deg |
|
|
|
- * post-fix: 5 min in 100% MeOH
- ** post-fix: 10 min in 4 deg Acetone and air dry, do not use any detergents
- ** post-fix: 10 min in 4 deg Acetone and air dry, do not use any detergents
References
- Aroian, RV et al. (1997) EMBO 16:1541-1549
- Guo, S and Kemphues, KJ (1995) Cell 81:611-620
- Etemad-Moghadam, B et al (1995) Cell 83:743-752
- Strome, S and Wood, WB (1983) Cell 35:15-25
