Immunization Protocol for Monoclonal Antibody Production
Antigens:
Proteins: 10-100 µg
Cells: 0.5 - 5.0 x107
Adjuvant:
Complete Freunds Adjuvant
None
Schedule:
|
Day |
Manipulation |
Adjuvant |
Site |
| 0 | Primary Immunization | CFA | s.c. |
| 14 | Boost #1 | IFA | s.c. |
|
24 |
Boost #2 | IFA | s.c. |
| 35 | Boost #3 | IFA | i.v. |
| 42 | Bleed and test for antibody | ||
| 45 | Boost #4 | IFA | i.v. |
| 66+ | 3-4 week rest followed by i.v. or i.p. boost | Saline/PBS | i.v. |
| 69 | fusion 3d after i.v. boost or 4d after i.p. boost | -- | i.v. |
