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Solution Ι
Cell resuspension solution (50 mM Tris-HCl, pH 7.5, 10 mM EDTA, RNase A 100 μg/ml)
1 M Tris-HCl (pH 7.6) 2.5 ml
0.25 M EDTA 2.0 ml
ddH2O 45 ml
sterile by autoclave
add 1% RNase 0.5ml
store at room temperature
Solution Ⅱ
Cell lysis solution (0.2 N NaOH, 1% SDS)
Mix 0.4 N NaOH and 2% SDS in same volume.
Solution Ⅲ
Neutralization solution (1.32 M potassium acetate, pH 5.2)
5 M potassium acetate 13.2 ml
Hac 27 ml
adjust pH to 5.2
add ddH2O to 50 ml
sterile by autoclave
store at room temperature
RNaseA: 10mg/ml溶于TE中，在沸水中煮10－30分钟之后分成小份储存于－20℃
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