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Calcuim assay
Review |
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Recombinant aequorin and green fluorescent protein as valuable tools in the study of cell signalling
Anna CHIESA etl.
Biochem. J. (2001) 355, 1±12 |
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本文论述了基于重组水母发光蛋白和绿色荧光蛋白的BRET技术在细胞信号传导，特别是钙离子介导的信号传导途径上的应用。
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Methods Article |
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Aequorin-based measurements of intracellular Ca2+-signatures in plant cells
Biol. Proced. Online 2002;4:105-118 |
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钙离子作为植物信号传导途径中一个主要的二级信使，越来越关注钙离子在植物细胞受到一个刺激后特定反应模式中的某些作用。在本文描述了用一种基于水母发光蛋白（aequorin）的非侵入性技术来研究植物细胞不同部分（胞质和核）对生物的（elicitors）和非生物的（osmotic shocks）刺激而引发的钙离子变化情况。
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Calcium measurement in living filamentous fungi expressing codon-optimized aequorin
G. Nelson,O. Kozlova-Zwinderman,et.al
Molecular Microbiology (2004) 52 (5), 1437–1450 |
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因为简单易行的方法的缺乏，钙离子信号途径在丝状真菌中的情况了解的非常少。本文发展了一种基于重组水母发光蛋白的方法来进行此研究。通过密码子优化，高水平表达水母蛋白的Neurospora crassa, Aspergillus niger 和Aspergillus awamori 菌株已得到。
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Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system
JUNKO NAKAJIMA-SHIMADA, HIDETOSHI IIDA, FREDERICK I. Tsujit, AND YASUHIRO ANRAKU
Proc Natl Acad Sci U S A. 1991 August 1; 88(15): 6878–6882
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Transgenic AEQUORIN reveals organ-specific cytosolic Ca2+ responses to anoxia and Arabidopsis thaliana seedlings
John C. Sedbrook, Paul j. Kronebusch, Cary C. Borisy, Anthony j. Trewavas, and Patrick H. Masson
Plant Physiol. 1996 May; 111(1): 243–257
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Subcellular Imaging of Intramitochondrial Ca2+ with Recombinant Targeted Aequorin: Significance for the Regulation of Pyruvate Dehydrogenase Activity
Proc Natl Acad Sci U S A. 1996 May 28; 93(11): 5489–5494
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Mechanisms of Signal Transduction |
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Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells
David Lecourieux, Christian Mazars, Nicolas Pauly, Raoul Ranjeva, and Alain Pugin
The Plant Cell 2002, Vol. 14, 2627-2641 |
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通过能稳定表达水母发光蛋白的烟草悬浮细胞来研究分析植物细胞的保护启动后细胞质中游离钙离子的浓度变化。钙离子浓度的延滞时间、峰谷、强度和持续时间都有不同。
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Different circadian oscillators control Ca2+ fluxes and Lhcb gene expression
JIQING SAI AND CARL HIRSCHIE JOHNSON
Plant Biology，1999，Vol. 96, Issue 20, 11659-11663 |
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昼夜节律控制很多的生命过程，但是控制这些过程的机理大多数是未知的。基于细胞质中的游离钙离子水平控制了植物中Lhcb基因的表达，此文通过测试游离钙离子的昼夜节律是否与Lhcb基因的表达节奏有关。
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Distinct Calcium Signaling Pathways Regulate Calmodulin Gene Expression in Tobacco
Arnold H. van der Luit, Claudio Olivari, Ann Haley, Marc R. Knight, and Anthony J. Trewavas
Plant Physiology, 1999, Vol. 121, pp. 705-714 |
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冷刺激和风激发了Ca2+的转移，通过水母发光蛋白的转基因烟草来研究其中的关系和可能的机理。
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Mitochondrial and Cytosolic Calcium Dynamics Are Differentially Regulated in Plants
David C. Logan and Marc R. Knight
Plant Physiology, 2003, Vol. 133, pp. 21-24 |
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因为技术困难，很少有人注意植物细胞信号传导过程中线粒体钙离子的作用。体内测试线粒体中的钙离子浓度([Ca2+]m)是比较困难的，这儿通过能稳定表达水母发光蛋白的拟南芥，并定点到线粒体中以用于研究分析植物线粒体中钙离子变化和揭示[Ca2+]m 的独立调节。
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Stimulation of the blue light phototropic receptor NPH1 causes a transient increase in cytosolic Ca2+
Gideon Baum, Joanne C. Long, Gareth I. Jenkins, and Anthony J. Trewavas
Plant Biology，Vol. 96, Issue 23, 13554-13559,1999 |
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蓝光调节了植物生长、发育，调节三个光受体－CRY1、CRY2和NPH1。但是对这三个受体的转导途径了解很少。通过水母发光蛋白的转基因植物来研究这些受体是如何调节细胞内钙离子的。
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Temperature sensing by plants: the primary characteristics of signal perception and calcium response
The Plant Journal，Volume 18 Issue 5 Page 491，1999 |
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冷会立即激发植物细胞内自由钙离子浓度的升高。本文利用水母发光蛋白转基因植物来研究三个潜在的机制：称之为sensing, sensitisation和desensitisation。
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The Calcium Rhythms of Different Cell Types Oscillate with Different Circadian Phases
Nicola T. Wood, Ann Haley, Martine Viry-Moussa, Carl H. Johnson, Arnold H. van der Luit4, and Anthony J. Trewavas
Plant Physiology, 2001, Vol. 125, pp. 787-796
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Reorientation of Seedlings in the Earth's Gravitational Field Induces Cytosolic Calcium Transients
Christoph Plieth and Anthony J. Trewavas
Plant Physiol. 2002 June 1; 129(2): 786–796
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Nod Factors and Chitooligomers Elicit an Increase in Cytosolic Calcium in Aequorin-Expressing Soybean Cells
Joachim Muller, Christian Staehelin, Zhi-Ping Xie, Gabriele Neuhaus-Url, and Thomas Boller
Plant Physiol. 2000 October 1; 124(2): 733–740
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Primary Inhibition of Hypocotyl Growth and Phototropism Depend Differently on Phototropin-Mediated Increases in Cytoplasmic Calcium Induced by Blue Light
Kevin M. Folta, Erin J. Lieg, Tessa Durham, and Edgar P. Spalding
Plant Physiol. 2003 December; 133(4): 1464–1470
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Effects of PMCA and SERCA pump overexpression on the kinetics of cell Ca2+ signaling
Kevin M. Folta, Erin J. Lieg, Tessa Durham, and Edgar P. Spalding
The EMBO Journal, Vol. 19, No. 18 pp. 4926-4935, 2000
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Wind-induced plant motion immediately increases cytosolic calcium
MARC R. KNIGHT, STEVEN M. SMITH, AND ANTHONY J. TREWAVAS
Proc Natl Acad Sci U S A. 1992 June 1; 89(11): 4967–4971
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Protection against Heat Stress-Induced Oxidative Damage in Arabidopsis Involves Calcium, Abscisic Acid, Ethylene, and Salicylic Acid
Jane Larkindale and Marc R. Knight
Plant Physiol. 2002 February 1; 128(2): 682–695
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Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation
Heather Knight, Anthony J. Trewavas, and Marc R. Knight
Plant Cell. 1996 March; 8(3): 489–503
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Ca(2+)-activated anion channels and membrane depolarizations induced by blue light and cold in Arabidopsis seedlings
Bryan D. Lewis, CEOrge Karlin-Neumann, Ronald W. Davis, and Edgar P. Spalding
Plant Physiol. 1997 August; 114(4): 1327–1334
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Convergence of Calcium Signaling Pathways of Pathogenic Elicitors and Abscisic Acid in Arabidopsis Guard Cells
Birgit Klu sener, Jared J. Young, Yoshiyuki Murata, Gethyn J. Allen, Izumi C. Mori,
Veronique Hugouvieux, and Julian I. Schroeder
Plant Physiol. 2002 December 1; 130(4): 2152–2163
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Use of Recombinant Aequorin to Study Calcium Homeostasis and Monitor Calcium Transients in Response to Heat and Cold Shock in Cyanobacteria
Ignacio Torrecilla, Francisco Leganes, Ildefonso Bonilla, and Francisca Fernandez-Pinas
Plant Physiol. 2000 May 1; 123(1): 161–176
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Dark-Stimulated Calcium Ion Fluxes in the Chloroplast Stroma and Cytosol
Jiqing Sai and Carl Hirschie Johnson
Plant Cell. 2002 June; 14(6): 1279–1291
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phot1 and phot2 mediate blue light-induced transient increases in cytosolic Ca2+ differently in Arabidopsis leaves
Akiko Harada, Tatsuya Sakai, and Kiyotaka Okada
Proc Natl Acad Sci U S A. 2003 July 8; 100(14): 8583–8588
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Effects of Mechanical Signaling on Plant Cell Cytosolic Calcium
Proc Natl Acad Sci U S A. 1995 May 9; 92(10): 4124–4128
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Measurement of cytoplasmic free Ca2+ concentration in rabbit aorta using the photoprotein, aequorin. Effect of atrial natriuretic peptide on agonist-induced Ca2+ signal generation
Yoh Takuwa and Howard Rasmussen
J Clin Invest. 1987 July; 80(1): 248–257
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Phytopathology |
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Expression of Recombinant Aequorin as an Intracellular Calcium Reporter in the Phytopathogenic Fungus Phyllosticta ampelicida
Brian D. Shaw, Olga Kozlova, Nick D. Read, B. Gillian Turgeon, and H. C. Hoch
Fungal Genetics and Biology 34, 207–215 (2001) |
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Phyllosticta ampelicida 孢子的发芽需要有底物的促发。已有研究工作证明在发芽孔处必须存在有外来游离钙离子。本文描述了通过转有水母发光蛋白基因的P. ampelicida 转基因菌株来监测细菌细胞质中游离钙离子的情况。
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Receptor-Mediated Increase in Cytoplasmic Free Calcium Required for Activation of Pathogen Defense in Parsley
Beatrix B. et al.
Plant Cell. 2000 August; 12(8): 1425–1440.
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Cell Biology |
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Mitochondrial Ca2+-induced Ca2+ Release Mediated by the Ca2+ Uniporter
Mayte Montero, Maria Teresa Alonso, Almudena Albillos,Javier Garcı´a-Sancho, and Javier Alvarez
Mol Biol Cell. 2001 Jan; 12(1): 63-71.
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Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level
Valerie Baubet, Herve Le Mouellic, Anthony K. Campbell, Estelle Lucas-Meunier, Philippe Fossier,and Philippe Bru let
Proc Natl Acad Sci U S A. 2000; 97(13): 7260–7265
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The Ca2+ concentration of the endoplasmic reticulum is a key determinant of ceramide-induced apoptosis: significance for the molecular mechanism of bcl-2 action
Paolo Pinton. et al.
EMBO J. 2001 June; 20(11): 2690–2701.
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Aequorin targeted to the endoplasmic reticulum reveals heterogeneity in luminal Ca++ concentration and reports agonist- or IP3-induced release of Ca++
Donald Button and Alec Eidsath
Mol Biol Cell. 1996 March; 7(3): 419–434
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Subcellular analysis of Ca2+ homeostasis in primary cultures of skeletal muscle myotubes
Marisa Brini, Francesca De Giorgi, et al
Mol Biol Cell. 1997 January; 8(1): 129–143
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Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells
David Lecourieux, Christian Mazars, Nicolas Pauly, Raoul Ranjeva, and Alain Pugin
Plant Cell. 2002 October; 14(10): 2627–2641
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Beat-to-beat oscillations of mitochondrial [Ca2+] in cardiac cells
David Lecourieux, Christian Mazars, Nicolas Pauly, Raoul Ranjeva, and Alain Pugin
EMBO Journal, Vol. 20, No. 17 pp. 4998-5007, 2001
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The Calcium Rhythms of Different Cell Types Oscillate with Different Circadian Phases
Nicola T. Wood, Ann Haley, Martine Viry-Moussaıd, Carl H. Johnson, Arnold H. van der Luit, and Anthony J. Trewavas
Plant Physiol. 2001 February 1; 125(2): 787–796
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Molecular Biology |
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Bioluminescence of the Ca2+-binding photoprotein aequorin after cysteine modification
KouICHI KUROSE, SATOSHI INOUYE, YOSHIYUKI SAKAKI, AND FREDERICK. TSUji
Proc Natl Acad Sci U S A. 1989 January; 86(1): 80–84
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Signal amplification system for DNA hybridization assays based on in vitro expression of a DNA label encoding apoaequorin
Stephanie R. White and Theodore K. et al
Nucleic Acids Res. 1999 October 1; 27(19): e25
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Differential mRNA Degradation of Two β-Tubulin Isoforms Correlates with Cytosolic Ca2+ Changes in Glucan-Elicited Soybean Cells
Chantal Ebel, Lourdes Gomez Gomez, Anne-Catherine Schmit, Gabriele Neuhaus-Url, and Thomas Boller
Plant Physiol. 2001 May 1; 126(1): 87–96
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Cloning and sequence analysis of cDNA for the luminescent protein aequorin
SATOSHI INOUYE, et al
Proc Natl Acad Sci U S A. 1985 May; 82(10): 3154–3158
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Extraction of Renilla-type luciferin from the calcium-activated photoproteins aequorin, mnemiopsin, and berovin
WILLIAM W. WARD AND MILTON J. CORMIER
Proc Natl Acad Sci U S A. 1975 July; 72(7): 2530–2534
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Clinical Medicine |
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Role of intracellular sodium in the regulation of intracellular calcium and contractility. Effects of DPI 201-106 on excitation-contraction coupling in human ventricular myocardium
Judith K. Gwathmey, Mara T. Slawsky, G. Maurice Bnggs, and James P. Morgan
J Clin Invest. 1988 November; 82(5): 1592–1605
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Sodium/calcium exchange modulates intracellular calcium overload during posthypoxic reoxygenation in mammalian working myocardium. Evidence from aequorin-loaded ferret ventricular muscles
Yasuki Kihara, Shigetake Sasayama, Moriaki Inoko, and James P. Morgan
J Clin Invest. 1994 March; 93(3): 1275–1284
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Glucose-stimulated insulin secretion correlates with changes in mitochondrial and cytosolic Ca2+ in aequorin-expressing INS-1 cells
Eleanor D. Kennedy, Rosario Rizzuto, Jean-Marc Theler, William-F. Pralong, Carlo Bastianutto, Tullio Pozzan, and Claes B. Wollheim
J Clin Invest. 1996 December 1; 98(11): 2524–2538
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Mechanism of myocardial contractile depression by clinical concentrations of ethanol. A study in ferret papillary muscles
Thomas Guarnieri and Edward G. Lakattat
J Clin Invest. 1990 May; 85(5): 1462–1467
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Magnesium relaxes arterial smooth muscle by decreasing intracellular Ca2+ without changing intracellular Mg2+
Elizabeth K. Gilbert DAngelo, Harold A. Singer, and Christopher M. Rembold
J Clin Invest. 1992 June; 89(6): 1988–1994
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Effect of common agonists on cytoplasmic ionized calcium concentration in platelets. Measurement with 2-methyl-6- methoxy 8-nitroquinoline (quin2) and aequorin
J. Anthony Ware, Peter C. Johnson, Marianne Smith, and Edwin W. Salzman
J Clin Invest. 1986 March; 77(3): 878–886
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Differential effect of DPI 201-106 on the sensitivity of the myofilaments to Ca2+ in intact and skinned trabeculae from control and myopathic human hearts
Roger J. Hajjar, Judith K. Gwathmey, G. Maurice Bnggs, and James P. Morgan
J Clin Invest. 1988 November; 82(5): 1578–1584.
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Developmental Biology |
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Imaging of multicellular large-scale rhythmic calcium waves during zebrafish gastrulation
EDWIN GILLAND, ANDREW L. MILLER, ERIC KARPLUS, ROBERT BAKER, AND SARAH E. WEBB
Proc Natl Acad Sci U S A. 1999 January 5; 96(1): 157–161
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