Lowry Protein Assay

作者：Protocol    实验频道来源：本站原创    点击数：    更新时间：2004-7-21

Lowry Protein Assay  reagents 2% Na2CO3 in 0.1 N NaOH 2% NaK Tartrate 1% CuSO4•5H2O Mixture before use:  25.00 ml of 1                               0.25 ml of 2                               0.25 ml of 3  2N Folin reagent (yellow in color)  Standard: Given BSA 1 mg/ml  procedure Transfer sample containing up to 40 µg of protein to test tubes and bring volume up to 200 µL with H2O. Add 1 mL of mixture and mix well with the Vortex. Incubate for 10 minutes.at Room Temperature. Add 50 µL of Folin reagent, mix well with Vortex. Incubate for 30 minutes at Room Temperature. Read at 540 nm against an H2O blank. 处理 SSI 文件时出错