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- Autoradiography
(William H. Heidcamp)
This procedure involves the
incorporation of a radioactive substance into a cell, and
subsequent detection of that material through the use of a
photographic emulsion. The primary source used for cell biology is
an organic molecule containing tritium, the radioactive form of
hydrogen. Radioactive carbon, phosphorous and iodine are
occasionally utilized, but tritium has inherently more resolution
than any of the others
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- Disruption by Fusion PCR (David
Amberg and Ellen Beasley)
1) In separate PCR reactions, amplify
the 5' and 3' ends of the gene of interest with primers about 200
bases apart. Primer 2 should begin with 24 nts complementary to
the m13 forward primer(GTC GTG ACT GGG AAA ACC CTG GCG) and primer
3 should begin wit h 24 nts complementary to the m13 reverse
primer (TCC TGT GTG AAA TTG TTA TCC GCT). PCR amplify the marker
using the m13 forward and reverse primers.
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- In-Gel
Digest Protocol (Siuzdak Lab)
Procedure
for the Micropreparation of Internal Protein Fragments for Amino
Acid Sequencing
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Plant related
Lipid
Protocols
Animal
Techniques
Drosophila Related Protocols
C.
Elegans Protocols
Biochemistry
- Quantitation of Epitope-tagged GPCR's by Biotin
Labeling(Von Zastrow lab)
This
protocol is specifically designed for measuring the
agonist-induced degradation of FLAG and HA-tagged GPCRs expressed
on the plasma membrane of Human Embryonic Kidney (HEK) 293
cells.
- Flow
Cytometry Assay to measure Internalization of GPCR's
(Von Zastrow lab)
Internalization of
receptor is measured as the reduction of receptors on the plasma
membrane surface. Surface receptors are labeled with fluoresent
antibodies which is in turn measured using a flow cytometer. The
antibodies recognize an epitope engineered onto the N-terminus of
the GPCR.
Methylation
Analysis
Xenopus Egg
Extractology
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版权所有http://www.bioon.com,2001-2002 安徽中医学院 邮政编码：230038
