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- In
Vitro Production of Bovine Embryos (P.J. Hansen
Lab, Dept. of Animal Sciences, University of
Florida)
This protocol describe procedures for in vitro
production [IVP; i.e. in vitro maturation (IVM),
in vitro fertilization (IVF), and in vitro
culture (IVC)]of embryos
- Mini-Chamber
for regulating gaseous environment during
culture (P.J. Hansen Lab, Dept. of
Animal Sciences, University of Florida)
A simple and inexpensive chamber for
regulating gaseous environment of small
culture plates, such as those used for culture
of preimplantation embryos, can be constructed
using disposable media-filtration devises such
as Corning’s 115-ml system. The
following is a description of how to make such
a device
- Cell
number counting in preimplantation embryos
(P.J. Hansen Lab, Dept. of Animal
Sciences, University of Florida)
A simple procedure for determining cell number
in preimplantation embryos. While the
procedure is written for fixed embryos,
fixation is optional since the Hoechst 33342
and 4',6-diamidino-2-phenylindole,
dihydrochloride (DAPI) penetrate living cells.
The fixation step is useful when embryos will
be stored for several weeks before
determination of cell number
- TUNEL
procedure for preimplantation embryos (P.J. Hansen
Lab, Dept. of Animal Sciences, University of
Florida)
A protocol for detecting cell death using
TUNEL.
- Lineage
Analysis Using Retrovirus Vectors (Cepko
Lab, Department of Genetics, Harvard Medical
School and Howard Hughes Medical
Institute,)
Describe the use of replication-incompetent
retroviral vectors for the analysis of lineal
relationships in developing vertebrate
tissues.
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