Nature Medicine：液滴检测禽流感

生物谷

    也许在不久的将来，禽流感的检测将非常容易。在9月在线出版的《自然—医学》期刊上，Juergen  Pipper和同事描述了一种廉价、快速又有效的液滴检测方法，这种方法能够在30分钟的时间内从一份鼻咽喉抹检样品中直接检测出禽流感病毒H5N1。新方法还可应用于SARS、艾滋病和乙肝等疾病病毒的检测。

    在流感流行的威胁中，快速的围堵政策依赖于对第一例病例的快速诊断和鉴别。但在公共卫生资源受限的国家，能否使用常规检测方法是一个问题。因此，低成本、方便使用的分析方法更有优势。

    新系统利用了这样一种液滴，这种液滴含有可自动分离、纯化和浓缩病毒RNA的颗粒。新方法的灵敏度与其他方法相当，但速度要快100倍，价格也更便宜。此外，这种方法还可应用于其他感染性病毒的检测，也可利用血液、尿液或唾液等体液进行检测。（科学时报）

原始出处:

Nature Medicine 13, 1259 - 1263 (2007)
Published online: 23 September 2007 | doi:10.1038/nm1634

Catching bird flu in a droplet

Juergen Pipper1, Masafumi Inoue2, Lisa F-P Ng3, Pavel Neuzil1,4, Yi Zhang1,4 & Lukas Novak1,4

It is assumed that a timely mass administration of antiviral drugs, backed by quarantines and social distancing, could contain a nascent influenza epidemic at its source, provided that the first clusters of cases were localized within a short time. However, effective routine surveillance may be impossible in countries lacking basic public health resources. For a global containment strategy to be successful, low-cost, easy-to-use handheld units that permit decentralized testing would be vital. Here we present a microfluidic platform that can detect the highly pathogenic avian influenza virus H5N1 in a throat swab sample by using magnetic forces to manipulate a free droplet containing superparamagnetic particles. In a sequential process, the viral RNA is isolated, purified, preconcentrated by 50,000% and subjected to ultrafast real-time RT-PCR. Compared to commercially available tests, the bioassay is equally sensitive and is 440% faster and 2,000–5,000% cheaper.

Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669.
Institute of Molecular and Cell Biology, 61 Biopolis Drive, The Proteos, Singapore 138673.
Genome Institute of Singapore, 60 Biopolis Street, The Genome, #02-01, Singapore 138672.
Present addresses: Institute of Microelectronics, 11 Science Park Road, Science Park II, Singapore 117685 (P.N); Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA (Y.Z.); Czech Technical University, Technická 2, CZ-16627 Prague 6, Czech Republic (L.N.).

Correspondence to: Juergen Pipper1 e-mail: jpipper@ibn.a.star.edu.sg

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