神经干细胞能在三维纳米纤维上选择性分化
生物谷报道：干细胞和祖细胞定向分化是目前研究热点之一，而结合三维（立体）材料进行定向分化，则被认为是组织工程上最有希望的前景。过去多采用高分子可降解的多聚物，同时附上不同的药物，使胚胎干细胞能定向分化，且能形成三维结构，这一成果发表在去年10月分的PNAS上(见生物谷内相关报道)。而这一自主性体温调节研究成果则是将纳米纤维上附接上两性肽分子,从而使神经祖细胞选择性分化,这一研究成果开创了材料与定向分化结合的新篇章,同时也为组织工程开辟了新的领域和方向。
Selective Differentiation of Neural Progenitor Cells by High-Epitope Density Nanofibers
Science, Vol. 303, Issue 5662, 1352-1355, February 27, 2004
Neural progenitor cells were encapsulated in vitro within a three-dimensional network of nanofibers formed by self-assembly of peptide amphiphile molecules. The self-assembly is triggered by mixing cell suspensions in media with dilute aqueous solutions of the molecules, and cells survive the growth of the nanofibers around them. These nanofibers were designed to present to cells the neurite-promoting laminin epitope IKVAV at nearly van der Waals density. Relative to laminin or soluble peptide, the artificial nanofiber scaffold induced very rapid differentiation of cells into neurons, while discouraging the development of astrocytes. This rapid selective differentiation is linked to the amplification of bioactive epitope presentation to cells by the nanofibers.
Full Text of this Article
PDF Version of this Article
Supporting Online Material
- 众说风云 (已有0条评论)
