韩国科学家从胚泡中克隆出纯化的人的胚胎干细胞
生物谷报道：最近如何获得纯化的人的胚胎干细胞成为研究中的热点，有从丢弃的胚胎中获得，有的通过干细胞克隆中胚泡后，再从胚泡中纯化，也有通过核移植手段获得人的干细胞，而韩国最近一系列研究颇引人注目。这篇文章在今天出版的Science上以封面文章形式报道，希望大家看看。
Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.
Original article:
Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst.Woo Suk Hwang, Young June Ryu, Jong Hyuk Park, Eul Soon Park, Eu Gene Lee, Ja Min Koo, Hyun Yong Jeon, Byeong Chun Lee, Sung Keun Kang, Sun Jong Kim, Curie Ahn, Jung Hye Hwang, Ky Young Park, Jose B. Cibelli, and Shin Yong Moon
Science 12 March 2004: 1669-1674. Published online 12 February 2004 [DOI: 10.1126/science.1094515] (in Science Express Reports )
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