胰岛素样生长因子-I调节髁突软骨细胞凋亡
内源性胰岛素样生长因子-I（IGF-I）可影响髁突软骨的生长与发育。但是，IGF-I对细胞存活的关键作用并未明确。
日本东京医科大学的Yokota T及其同事在最近的研究中对以下假设进行了检验，即IGF-I可能对下颌骨髁突软骨细胞的存活具有调节作用。
研究人员将取自12日龄大鼠的下颌骨髁突在含有IGF-I反义寡核苷酸（AS-ODN）的培养基中培养1、3和5天。实时RT-PCR分析显示，AS-ODN组中IGF-I和IGF结合蛋白（IGFBP）3的mRNA水平显著降低。在培养3天后观察未分化间充质细胞层中坏死细胞的数量。这些细胞呈TUNEL阳性，经电镜观察证实为凋亡细胞。免疫印迹显示AS-ODN组激活型caspase3表达增加。
Yokota等总结认为，这些结果可能提示下颌骨髁突未分化间充质细胞层中细胞的存活需要IGF-I。（医学空间）
生物谷推荐原始出处：
J Dent Res 87(2):159-163, 2008
RESEARCH REPORT
Biological
Insulin-like Growth Factor I Regulates Apoptosis in Condylar Cartilage
1 Section of Maxillofacial Orthognathics, Department of Maxillofacial Restoration, Division of Maxillofacial/Neck Reconstruction, and
2 Section of Pharmacology, Department of Hard Tissue Engineering, Division of Bio-Matrix, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan; and
3 Oral Histology, Graduate School of Dentistry, Health Science University of Hokkaido, Japan
* corresponding author, s-suzuki.mort@tmd.ac.jp
Endogenous insulin-like growth factor-I (IGF-I) is known to affect the growth and development of condylar cartilage. However, the critical effect of IGF-I on cell survival is still unknown. We hypothesized that endogenous IGF-I could regulate the survival of cells of the mandibular condylar cartilage. Mandibular condyles dissected from 12-day-old rats were cultured for 1, 3, and 5 days in medium containing antisense oligodeoxynucleotide (AS-ODN) for IGF-I. Real-time RT-PCR analysis showed that the levels of IGF-I and IGF binding protein (IGFBP)3 mRNAs in the AS-ODN group were significantly decreased. After 3 days’ culture, the number of necrotic cells was observed in the undifferentiated mesenchymal cell layer. These cells were TUNEL-positive and confirmed to be apoptotic by electron microscopic observation. Immunoblotting revealed that expression of cleaved caspase3 was increased with AS-ODN. These results may suggest that the cells in the undifferentiated mesenchymal cell layer of the mandibular condyle require IGF-I for survival.
KEY WORDS: IGF-I • mandibular condylar cartilage • rat • apoptosis • antisense-ODN
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