生物物理所赵保路新文章：绿茶能够保护脑细胞
生物谷报道：喝茶是东方人的传统，中国也是茶的故乡。而绿茶被认为是健康圣品。在
由于绿茶饮品在世界享有很高的声誉，人们对它在健康方面的有益效果相当关注。Biological Psychiatry的编辑John H. Krystal博士提醒说，许多有关绿茶对健康的种种好处的说法并没有在严格的临床研究中得到证实。因此，中国科学家在动物模型中证实绿茶多酚的神经保护机制是战胜帕金森症的一个好的开端。
赵保路研究员，1981年，中国科学院研究生院获硕士学位。1985-1997年先后到美国俄亥俄州立大学、加州大学伯克立分学和英国国立食品研究所合作研究。1995年后任生物物理研究所研究员至今。现为亚洲自由基研究学会主席，中国生物物理学会自由基与医学专业委员会主任，衰老与抗衰老委员会副主任。
原始出处:
Biological Psychiatry
Volume 62, Issue 12, 15 December 2007, Pages 1353-1362
Neurodegenerative Disorders: Early Phenotypes, Neuroplasticity, and Progression
Protective Effects of Green Tea Polyphenols in the 6-OHDA Rat Model of Parkinson’s Disease Through Inhibition of ROS-NO Pathway
Shuhong Guoa, Jingqi Yana, b, Tangbin Yangd, Xianqiang Yange, Erwan Bezardc and Baolu Zhao
a, f, 
, 
aState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Academia Sinica, Beijing, China
bGraduate School of Chinese Academy of Sciences, Beijing, China
cNational Center for Scientific Research (The Centre National de la Recherche Scientifique), Bordeaux, France
dSpace Cell and Molecular Biology Laboratory, Institute of Space and Medico-Engineering, Beijing, China
eDepartment of Tea Science, Zhejiang University, Hangzhou, China
fE-Institutes of Shanghai Municipal Education Commission, China.
Received 22 November 2006; revised 11 April 2007; accepted 12 April 2007. Available online 12 July 2007.
Background
Nitric oxide (NO) and related pathways are thought to play an important role in the pathogenesis of Parkinson’s disease (PD). Our in vitro experiments suggested that green tea polyphenols (GTP) might protect dopamine neurons through inhibition of NO and reactive oxygen species (ROS).
Methods
Immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labeling assay, electron spin resonance spin trapping, enzyme linked immunosorbent assay, and molecular biological methods were used to investigate the effects of GTP in an unilateral 6-hydroxydopamine (6-OHDA)-treated rat model of PD.
Results
GTP treatment dose-dependently protected dopaminergic neurons by preventing from midbrain and striatal 6-OHDA-induced increase in 1) both ROS and NO levels, 2) lipid peroxidation, 3) nitrite/nitrate content, 4) inducible nitric oxide synthase, and 5) protein-bound 3-nitro-tyrosine. Moreover, GTP treatment dose-dependently preserved the free radical scavenging capability of both the midbrain and the striatum.
Conclusions
These results support the in vivo protection of GTP against 6-OHDA and suggest that GTP treatment might represent a neuroprotective treatment of PD.
Key Words: Electron spin resonance; natural antioxidants; neurodegenerative disease; nitric oxide; reactive oxygen species; stereology Address reprint requests to Professor Baolu Zhao, State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Academia Sinica, Beijing 100101, P.R. China
相关报道:
附：赵保路研究员近年来发表的主要论文：
1. Shuhong Guo, Jingqi Yan, Erwan Bezard, Tangbin Yang, Xianqiang Yang, Baolu Zhao*。Protective effects of green tea polyphenols in the 6-OHDA rat model of Parkinson's disease through inhibition of ROS-NO pathway。Biol Psychiat （XX,E1-9,2007 www.sobp.org/journal）。
2. Qiang Liu, Jie Zhang, Hua Zhu, Chuan Qin, Qi Chen，Baolu Zhao*. Dissecting the Signalling Pathway of Nicotine-Mediated Neuroprotection in a Mouse Alzheimer Disease Model. FASEB J 21,61-73，2007。
3. Jie Zhang, Qiang Liu Nian-Qing Liu, Fu-Liang Li, Qi Chen, Chuan Qin, Hua Zhu， Yu-Ying Huang, Wei He, Bao-Lu Zhao*.Nicotine reduces β-amyloidosis by regulating metal homeostasis. FASEB J 20，1212-1214，2006。
4. Yu-Xiang Xie, Erwan Bezard, Bao-Lu. Zhao*: Unraveling the receptor- independent neuroprotective mechanism in mitochondria. J. Biol Chem. 37，32405-32412，2005。
5. Haiyan Zeng, Qi Chen, Baolu Zhao*. Genistein ameliorated β-amyloid peptide -induced hippocampal neuronal apoptosis. Free Rad Biol Med 36, 180-188,2004
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