Nature Biotechnology：肌上皮细胞可治疗肌肉营养失调
生物谷报道：肌肉营养失调是肌肉萎缩症的一种情况，一项新研究显示，现存的肌肉祖细胞可用于治疗这类肌肉萎缩症，新研究发表在9月在线出版的《自然—生物技术》期刊上。
Johnny Huard和同事指出，与祖细胞(或卫星细胞)相比，某种类型的成人骨骼肌细胞具有更大的能力再生成肌肉组织。
新的祖细胞名为肌上皮细胞(myoendothelial cells)，它能表达出卫星细胞和内皮细胞的细胞表面标志。利用标注细胞表面标志的荧光标签抗体和一种荧光激活细胞分类仪器，Huard和同事很容易就将肌上皮细胞分离出来。在培养液中，这些细胞能够向肌肉、骨骼和软骨分化。对小鼠的研究显示，与卫星细胞和内皮细胞相比，肌上皮细胞能更有效地形成身体内的肌肉纤维：将1000个肌上皮细胞移植入骨骼肌肉受伤的免疫缺陷动物体内后，能产生89种肌肉纤维，而相同数量的内皮或卫星细胞却只能产生9种或5种肌肉纤维。
对肌上皮细胞安全性的试验表明，这种细胞没有形成肿瘤的倾向。（援引科学时报）
原始出处:
Nature Biotechnology
Published online: 2 September 2007 | doi:10.1038/nbt1334
Prospective identification of myogenic endothelial cells in human skeletal muscle
Bo Zheng1, Baohong Cao1, Mihaela Crisan1, Bin Sun1, Guangheng Li1, Alison Logar2, Solomon Yap1, Jonathan B Pollett1,3, Lauren Drowley1, Theresa Cassino1, Burhan Gharaibeh1, Bridget M Deasy1, Johnny Huard1,4,5 & Bruno Péault1,2,5
We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56+ myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56+ myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease.
- Stem Cell Research Center, Children's Hospital of Pittsburgh; Department of Orthopaedic Surgery, University of Pittsburgh Children's Hospital and School of Medicine, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, Pennsylvania 15213-2583, USA.
- Department of Pediatrics, University of Pittsburgh Children's Hospital and School of Medicine, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, Pennsylvania 15213-2583, USA.
- University of Pittsburgh Cancer Institute, University of Pittsburgh Children's Hospital and School of Medicine, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, Pennsylvania 15213-2583, USA.
- Departments of Molecular Genetics & Biochemistry and of Bioengineering, University of Pittsburgh Children's Hospital and School of Medicine, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, Pennsylvania 15213-2583, USA.
- McGowan Institute for Regenerative Medicine, University of Pittsburgh Children's Hospital and School of Medicine, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, Pennsylvania 15213-2583, USA.
Correspondence to: Johnny Huard1,4,5 e-mail: jhuard@pitt.edu
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