ACRCCM：呼出气冷凝液检测可助诊肺癌
意大利福贾大学Carpagnano等的研究表明，在从非小细胞肺癌(NSCLC)患者呼出气冷凝液提取的DNA中，存在与其肺癌组织一致的微卫星改变，因而呼出气冷凝液检测有助于NSCLC的检出。相关论文发表在《美国呼吸和重症护理医学杂志》上。
研究纳入59例接受组织学诊断的临床可疑肺癌受试者，最终41例被诊断为NSCLC，18例为非肿瘤患者。研究者提取其全血、呼出气冷凝液和活检肺组织的DNA，对位于染色体3p的5个微卫星进行了分析。
结果显示，NSCLC患者呼出气冷凝液的微卫星改变显著多于对照者。对于每例NSCLC患者来说，其肿瘤组织和呼出气冷凝液DNA的微卫星改变(包括杂合性缺失和微卫星不稳定)高度一致;同样，在非肿瘤对照者中，其呼出气冷凝液DNA中的微卫星改变，也可反映其肺组织DNA的微卫星改变。此外，无论是NSCLC患者还是对照者，吸烟都可增加微卫星改变。
该研究提示，呼出气冷凝液检测可能是一种简单、无创的检测手段，可以检出存在微卫星改变的高危个体。（来源：中国医学论坛报）
生物谷推荐原始出处：
（American Journal of Respiratory and Critical Care Medicine），Vol 177. pp. 337-341, (2008)，Giovanna E. Carpagnano, Angelo Paradiso
3p Microsatellite Signature in Exhaled Breath Condensate and Tumor Tissue of Patients with Lung Cancer
1 Institute of Respiratory Disease, University of Foggia, Foggia, Italy; 2 Fondazione Salvatore Maugeri, Care and Research Institute, Cassano delle Murge, Bari, Italy; 3 Institute of Respiratory Disease, University of Bari, Bari, Italy; 4 Department of Thoracic Surgery, San Paolo Hospital, Bari, Italy; 5 ISPA, CNR, Bari, Italy; and 6 Clinical Experimental Oncology Laboratory, National Cancer Institute, Bari, Italy
Correspondence and requests for reprints should be addressed to Giovanna Elisiana Carpagnano, M.D., Ph.D., Via De Nicolò 5, 70121 Bari, Italy. E-mail: ge.carpagnano@unifg.it
Rationale: Our group has recently demonstrated the possibility of studying microsatellite alterations (MAs) of 3p in the DNA of exhaled breath condensate (EBC) of patients with non–small cell lung cancer (NSCLC).
Objectives: To verify whether MAs analyzed in DNA from EBC reflect a profile of alterations present in tumor tissue of NSCLC.
Methods: Fifty-nine subjects undergoing histologic diagnosis for clinical suspicion of lung cancer entered the study: 41 were found to have NSCLC and 18 to have nonneoplastic diseases. All subjects underwent allelotyping on DNA from whole blood, EBC, and lung tissue removed for histologic diagnosis by analyzing a panel of five microsatellites located in chromosomal region 3p. Results obtained from DNA of the three biological sites and nonneoplastic tissues from controls were compared.
Measurements and Main Results: MAs in DNA from tumor tissues and EBC of each patient with cancer presented an overlapping profile of loss of heterozygosity and microsatellite instability. An MA profile of DNA of lung tissue reflecting the DNA of EBC profile from controls was also confirmed. Smoking status was associated with the presence of MAs in patients with NSCLC and in control subjects.
Conclusions: We demonstrated that MAs in DNA from EBC of patients with NSCLC are significantly more frequent than in control subjects. More interesting, the MA profile of DNA from EBC corresponds to that from lung cancer tissue of each patient with NSCLC.
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