过程工程所制备出尺寸均一的自发荧光天然多糖微球
中科院过程工程研究所生化工程国家重点实验室博士研究生魏炜、助研王连艳等首次制备出了自发荧光的天然多糖微球，目前已广泛的应用于生物学研究领域，该研究得到了国家自然科学基金委、科技部和中国科学院的支持，并申请了国家发明专利。相关研究成果发表在Advanced Functional Materials( 2007, 17:3153)上，作为该期刊的封面文章。
传统的荧光微球以聚苯乙烯为原料，必须通过包埋荧光染料才能具有荧光性质。由于制备工艺复杂，生物兼容性差，存在着荧光信号弱、染料泄漏等问题。魏炜、王连艳等发现天然海洋多糖微球用特殊的交联剂交联后，微球已经具有明显的自发荧光现象，无需另外包埋荧光染料，且可以通过交联剂结构和交联技术的调节，制备不同颜色和不同发光强度的荧光微球。他们将这一发现与微孔膜乳化技术相结合，制备出了尺寸均一、颜色可调并且粒径可控、能自发荧光的壳聚糖微球，成功地用于生物示踪剂和药物靶向载体，将科学发现转化为技术发明，发展了一种新型的荧光微球及其制备方法。Advanced Functional Materials是功能材料领域的国际重要期刊，该期刊在发表魏炜、王连艳等人的论文时给予了推荐
“The autofluorescent microspheres may be useful in fluorescence assays as bright,
inexpensive, and stable probes for qualitative and quantitative studies of biological
interactions and drug delivery.”。（援引过程工程研究所）
原始出处:
Volume 17, Issue 16 , Pages 3153 - 3158
Published Online: 4 Oct 2007
|
Preparation and Application of Novel Microspheres Possessing Autofluorescent Properties
 |
|
W. Wei 1 2, L.-Y. Wang 1, L. Yuan 3, Q. Wei 1 2, X.-D. Yang 4, Z.-G. Su 1, G.-H. Ma 1 * |
| 1National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100080 Beijing (P.R. China) 2Graduate University of the Chinese Academy of Sciences, 100049 Beijing (P.R. China) 3Medical and Healthy Analytical Center, Peking University Health Science Center, 100083 Beijing (P.R. China) 4Department of Chemical Biology, State Key Laboratories of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, 100083 Beijing (P.R. China) |
| email: G.-H. Ma (ghma@home.ipe.ac.cn) |
*Correspondence to G.-H. Ma, 1National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100080 Beijing (P.R. China)
This project was supported by the National Nature Science Foundation of China under Contract Nos. 20 221 603, 20 536 050, and 20 376 082. The authors wish to thank Xing-Zhu Zhou, Yong-Hong Gu, Qing Xia, and Meng-Yao Li for their assistance. Supporting Information is available online from Wiley InterScience or from the author.
Funded by:
National Nature Science Foundation of China; Grant Number: 20 221 603, 20 536 050, 20 376 082
| Keywords |
| Crosslinking ?Fluorescence imaging ?Microspheres ?Polymeric materials |
| Abstract |
| Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The autofluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers. |
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