Genus-specific PCR for molecular identification of novel

Xiaoyang Zhi etc.…

微生物学报Acta Microbiologica Sinica

48(5)：644~650;4 May 2008

Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia

Xiaoyang Zhi1,Lingling Yang1,Yun Wang2,Shukun Tang1,Kai Lou2,Wenjun Li1*

(1Key Laboratory for Microbial Resources of Ministry of Education,Yunnan Institute of Microbiology,Yunnan University,Kunming 650091,China)
(2Xinjiang Institute of Microbiology,Xinjiang Academy of Agricultural Science,Urumqi 830091,China)

Supported by the Key Project of China National Programs for Fundamental Research and Development(2004CB719601),the National

Natural Science Foundation of China(30600001),the Key Project of Chinese Ministry of Education(206139)and the Program for New Century Excellent Talent in University(NCET)

*Corresponding author.Tel/Fax:+86-871-5033335;E-Mail:wjli@ynu.edu.cn,liact@hotmail.com

Abstract:

[Objective and Method]:For rapid identification of Nesterenkonia species,one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences.

[Results and Conclusion]:The genus specificity of these primers was validated with reference strains as well as with wild-type isolates.Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia.

全文下载：Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia

更多全文请查看链接：http://journals.im.ac.cn

声明：本文由《微生物学报》授权生物谷 www.bioon.com 网站发布，如需转载请直接与中国科学院微生物研究所期刊联合编辑部联系并支付相应费用，未经授权不得转载，若转载将付相应的法律责任。